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Literature summary extracted from

  • Thompson, L.D.; Brandon, L.D.; Nieuwlandt, D.T.; Daniels, C.J.
    Transfer RNA intron processing in the halophilic archaebacteria (1989), Can. J. Microbiol., 35, 36-42.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.16 spermidine required Haloferax volcanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.16 Mg2+
-
 Haloferax volcanii
4.6.1.16 monovalent cations at 50 mM Haloferax volcanii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.16 membrane soluble and membrane bound Haloferax volcanii 16020
-
4.6.1.16 soluble soluble and membrane bound Haloferax volcanii
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.16 tRNA precursor Haloferax volcanii
-
 a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.16 Haloferax volcanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.16 partial Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.16 tRNA precursor
-
 Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor substrate recognition at the exon-intron boundary region Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor precise and accurate excision of the intron from halobacterial tRNATrp precursor Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor does not require intact mature tRNA structure in the substrate Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?
4.6.1.16 tRNA precursor endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini Haloferax volcanii a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.16 37
-
 assay at Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.16 6 9
-
 Haloferax volcanii
4.6.1.16 7.5
-
 assay at Haloferax volcanii