Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, B.S.; Cropp, T.A.; Beck, B.J.; Sherman, D.H.; Reynolds, K.A.
    Biochemical evidence for an editing role of thioesterase II in the biosynthesis of the polyketide pikromycin (2002), J. Biol. Chem., 277, 48028-48034.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 expression in Escherichia coli BL21 as His-tagged protein Streptomyces venezuelae
3.1.2.20 expression in Escherichia coli BL21 as His-tagged protein Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 additional information Streptomyces venezuelae involved in the biosynthesis of the polyketide pikromycin ?
-
 ?
3.1.2.20 additional information Streptomyces venezuelae involved in the biosynthesis of the polyketide pikromycin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Streptomyces venezuelae Q9ZGI1 thioesterase II, gene pikAV
-
3.1.2.20 Streptomyces venezuelae Q9ZGI1 thioesterase II, gene pikAV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 recombinant from Escherichia coli BL21 as His-tagged enzyme, to homogeneity Streptomyces venezuelae
3.1.2.20 recombinant from Escherichia coli BL21 as His-tagged enzyme, to homogeneity Streptomyces venezuelae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acetyl-CoA + H2O poor substrate Streptomyces venezuelae CoA + acetate
-
 ?
3.1.2.2 acyl-CoA + H2O
-
 Streptomyces venezuelae CoA + a carboxylate
-
 ?
3.1.2.2 butyryl-[acyl-carrier protein] + H2O
-
 Streptomyces venezuelae butanoate + acyl-carrier protein
-
 ?
3.1.2.2 malonyl-CoA + H2O
-
 Streptomyces venezuelae CoA + malonate
-
 ?
3.1.2.2 additional information involved in the biosynthesis of the polyketide pikromycin Streptomyces venezuelae ?
-
 ?
3.1.2.2 n-butyryl-CoA + H2O
-
 Streptomyces venezuelae CoA + n-butanoate
-
 ?
3.1.2.2 propionyl-CoA + H2O
-
 Streptomyces venezuelae CoA + propionate
-
 ?
3.1.2.2 propionyl-[acyl-carrier protein] + H2O
-
 Streptomyces venezuelae propionate + acyl-carrier protein
-
 ?
3.1.2.20 acetyl-CoA + H2O poor substrate Streptomyces venezuelae CoA + acetate
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Streptomyces venezuelae CoA + a carboxylate
-
 ?
3.1.2.20 butyryl-[acyl-carrier protein] + H2O
-
 Streptomyces venezuelae butanoate + acyl-carrier protein
-
 ?
3.1.2.20 malonyl-CoA + H2O
-
 Streptomyces venezuelae CoA + malonate
-
 ?
3.1.2.20 additional information involved in the biosynthesis of the polyketide pikromycin Streptomyces venezuelae ?
-
 ?
3.1.2.20 n-butyryl-CoA + H2O
-
 Streptomyces venezuelae CoA + n-butanoate
-
 ?
3.1.2.20 propionyl-CoA + H2O
-
 Streptomyces venezuelae CoA + propionate
-
 ?
3.1.2.20 propionyl-[acyl-carrier protein] + H2O
-
 Streptomyces venezuelae propionate + acyl-carrier protein
-
 ?