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Literature summary extracted from

  • Larson, J.D.; Kolattukudy, P.E.
    Isolation and characterization of an acyl-CoA thioesterase from dark-grown Euglena gracilis (1985), Arch. Biochem. Biophys., 237, 27-37.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 bovine serum albumin activation Euglena gracilis
3.1.2.2 bovine serum albumin effect of stimulation increases as chain-length increases up to C-12 and decreases progressively as chain-length increases beyond C-12, inhibitory with octadecanoyl-CoA as substrate Euglena gracilis
3.1.2.2 hexadecanol activation, only with acyl-CoA of more than C-8 as substrates Euglena gracilis
3.1.2.2 Tetradecanol activation, only with acyl-CoA of more than C-8 as substrates Euglena gracilis
3.1.2.2 Triton X-100 activation, 0.005%, dodecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 bovine serum albumin activation Euglena gracilis
3.1.2.20 bovine serum albumin effect of stimulation increases as chain-length increases up to C-12 and decreases progressively as chain-length increases beyond C-12, inhibitory with octadecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 hexadecanol activation, only with acyl-CoA of more than C-8 as substrates Euglena gracilis
3.1.2.20 Tetradecanol activation, only with acyl-CoA of more than C-8 as substrates Euglena gracilis
3.1.2.20 Triton X-100 activation, 0.005%, dodecanoyl-CoA as substrate Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 bovine serum albumin octadecanoyl-CoA as substrate, stimulates with C-6 to C-12 CoA as substrates Euglena gracilis
3.1.2.2 diethyldicarbonate hydroxylamine reverses Euglena gracilis
3.1.2.2 diisopropylfluorophosphate
-
Euglena gracilis
3.1.2.2 dodecanoyl-CoA substrate inhibition Euglena gracilis
3.1.2.2 additional information no inhibition by NEM Euglena gracilis
3.1.2.2 O,O-Diethyl-O-(2,4,5-trichlorophenyl)-phosphate
-
Euglena gracilis
3.1.2.2 O,O-Diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate
-
Euglena gracilis
3.1.2.2 O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphorothionate i.e. Chloropyrifos Euglena gracilis
3.1.2.2 O,O-Dimethyl-O-(2,4,5-trichloro-1-phenyl)-phosphate
-
Euglena gracilis
3.1.2.2 O,O-Dimethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate i.e. Fospirate Euglena gracilis
3.1.2.2 p-hydroxymercuribenzoate weak inhibition Euglena gracilis
3.1.2.2 tetradecanoyl-CoA substrate inhibition Euglena gracilis
3.1.2.20 bovine serum albumin octadecanoyl-CoA as substrate, stimulates with C-6 to C-12 CoA as substrates Euglena gracilis
3.1.2.20 diethyldicarbonate hydroxylamine reverses Euglena gracilis
3.1.2.20 diisopropylfluorophosphate
-
Euglena gracilis
3.1.2.20 dodecanoyl-CoA substrate inhibition Euglena gracilis
3.1.2.20 additional information no inhibition by NEM Euglena gracilis
3.1.2.20 O,O-Diethyl-O-(2,4,5-trichlorophenyl)-phosphate
-
Euglena gracilis
3.1.2.20 O,O-Diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate
-
Euglena gracilis
3.1.2.20 O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphorothionate i.e. Chloropyrifos Euglena gracilis
3.1.2.20 O,O-Dimethyl-O-(2,4,5-trichloro-1-phenyl)-phosphate
-
Euglena gracilis
3.1.2.20 O,O-Dimethyl-O-(3,5,6-trichloro-2-pyridyl)-phosphate i.e. Fospirate Euglena gracilis
3.1.2.20 p-hydroxymercuribenzoate weak inhibition Euglena gracilis
3.1.2.20 tetradecanoyl-CoA substrate inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 additional information
-
additional information effect of pH on kinetic data Euglena gracilis
3.1.2.2 0.014
-
dodecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.2 0.017
-
hexadecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.2 0.024
-
tetradecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.2 0.027
-
decanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.2 0.034
-
octadecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.2 0.043
-
octanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.2 0.077
-
hexanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.20 additional information
-
additional information effect of pH on kinetic data Euglena gracilis
3.1.2.20 0.014
-
dodecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.20 0.017
-
hexadecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.20 0.024
-
tetradecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.20 0.027
-
decanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.20 0.034
-
octadecanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.20 0.043
-
octanoyl-CoA pH 7.0, 30°C Euglena gracilis
3.1.2.20 0.077
-
hexanoyl-CoA pH 7.0, 30°C Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
Euglena gracilis 5829
-
3.1.2.20 cytosol
-
Euglena gracilis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 37000
-
gel filtration Euglena gracilis
3.1.2.20 37000
-
gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 acyl-CoA + H2O Euglena gracilis
-
CoA + a carboxylate
-
?
3.1.2.2 acyl-CoA + H2O Euglena gracilis Z
-
CoA + a carboxylate
-
?
3.1.2.2 hexadecanoyl-CoA + H2O Euglena gracilis
-
CoA + hexadecanoate
-
?
3.1.2.20 acyl-CoA + H2O Euglena gracilis
-
CoA + a carboxylate
-
?
3.1.2.20 hexadecanoyl-CoA + H2O Euglena gracilis
-
CoA + hexadecanoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Euglena gracilis
-
Z, dark-grown
-
3.1.2.2 Euglena gracilis Z
-
Z, dark-grown
-
3.1.2.20 Euglena gracilis
-
Z, dark-grown
-
3.1.2.20 Euglena gracilis Z
-
Z, dark-grown
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 678fold Euglena gracilis
3.1.2.20 678fold Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 9.5
-
purified enzyme, lauroyl-CoA as substrate Euglena gracilis
3.1.2.20 9.5
-
purified enzyme, lauroyl-CoA as substrate Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O
-
Euglena gracilis CoA + a carboxylate
-
?
3.1.2.2 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Euglena gracilis CoA + a carboxylate
-
?
3.1.2.2 acyl-CoA + H2O
-
Euglena gracilis Z CoA + a carboxylate
-
?
3.1.2.2 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Euglena gracilis Z CoA + a carboxylate
-
?
3.1.2.2 decanoyl-CoA + H2O
-
Euglena gracilis CoA + decanoate
-
?
3.1.2.2 decanoyl-CoA + H2O
-
Euglena gracilis Z CoA + decanoate
-
?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Euglena gracilis CoA + dodecanoate
-
?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Euglena gracilis Z CoA + dodecanoate
-
?
3.1.2.2 hexadecanoyl-CoA + H2O
-
Euglena gracilis CoA + hexadecanoate
-
?
3.1.2.2 hexanoyl-CoA + H2O poor substrate Euglena gracilis CoA + hexanoate
-
?
3.1.2.2 n-butyryl-CoA + H2O poor substrate Euglena gracilis CoA + n-butanoate
-
?
3.1.2.2 octadecanoyl-CoA + H2O preferred substrate Euglena gracilis CoA + octadecanoate
-
?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Euglena gracilis CoA + octadecanoate
-
?
3.1.2.2 octanoyl-CoA + H2O
-
Euglena gracilis CoA + octanoate
-
?
3.1.2.2 octanoyl-CoA + H2O
-
Euglena gracilis Z CoA + octanoate
-
?
3.1.2.2 tetradecanoyl-CoA + H2O best substrate Euglena gracilis CoA + tetradecanoate
-
?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Euglena gracilis CoA + tetradecanoate
-
?
3.1.2.20 acyl-CoA + H2O
-
Euglena gracilis CoA + a carboxylate
-
?
3.1.2.20 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Euglena gracilis CoA + a carboxylate
-
?
3.1.2.20 decanoyl-CoA + H2O
-
Euglena gracilis CoA + decanoate
-
?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Euglena gracilis CoA + dodecanoate
-
?
3.1.2.20 hexadecanoyl-CoA + H2O
-
Euglena gracilis CoA + hexadecanoate
-
?
3.1.2.20 hexanoyl-CoA + H2O poor substrate Euglena gracilis CoA + hexanoate
-
?
3.1.2.20 n-butyryl-CoA + H2O poor substrate Euglena gracilis CoA + n-butanoate
-
?
3.1.2.20 octadecanoyl-CoA + H2O preferred substrate Euglena gracilis CoA + octadecanoate
-
?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Euglena gracilis CoA + octadecanoate
-
?
3.1.2.20 octanoyl-CoA + H2O
-
Euglena gracilis CoA + octanoate
-
?
3.1.2.20 tetradecanoyl-CoA + H2O best substrate Euglena gracilis CoA + tetradecanoate
-
?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Euglena gracilis CoA + tetradecanoate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 monomer 1 * 37000, SDS-PAGE Euglena gracilis
3.1.2.20 monomer 1 * 37000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 30
-
assay at Euglena gracilis
3.1.2.20 30
-
assay at Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 additional information
-
pH-optimum depends on chain-length of acyl-CoA substrate Euglena gracilis
3.1.2.2 4.5
-
hexanoyl-CoA or octanoyl-CoA as substrate Euglena gracilis
3.1.2.2 5
-
decanoyl-CoA as substrate Euglena gracilis
3.1.2.2 5.5
-
dodecanoyl-CoA as substrate Euglena gracilis
3.1.2.2 6 6.5 tetradecanoyl-CoA as substrate Euglena gracilis
3.1.2.2 6.5
-
hexadecanoyl-CoA as substrate Euglena gracilis
3.1.2.2 7
-
octadecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 additional information
-
pH-optimum depends on chain-length of acyl-CoA substrate Euglena gracilis
3.1.2.20 4.5
-
hexanoyl-CoA or octanoyl-CoA as substrate Euglena gracilis
3.1.2.20 5
-
decanoyl-CoA as substrate Euglena gracilis
3.1.2.20 5.5
-
dodecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 6 6.5 tetradecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 6.5
-
hexadecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 7
-
octadecanoyl-CoA as substrate Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 4.5 7 about 75% of maximal activity at pH 4.5 and about half-maximal activity at pH 7.0, dodecanoyl-CoA as substrate Euglena gracilis
3.1.2.2 6.2 8 about half-maximal activity at pH 6.2 and pH 8.0, octadecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 4.5 7 about 75% of maximal activity at pH 4.5 and about half-maximal activity at pH 7.0, dodecanoyl-CoA as substrate Euglena gracilis
3.1.2.20 6.2 8 about half-maximal activity at pH 6.2 and pH 8.0, octadecanoyl-CoA as substrate Euglena gracilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.2.2 additional information
-
enzyme is unstable to low pH Euglena gracilis
3.1.2.20 additional information
-
enzyme is unstable to low pH Euglena gracilis