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Literature summary extracted from

  • Naggert, J.; Narasimhan, M.L.; DeVeaux, L.; Cho, H.; Randhawa, Z.I.; Cronan, J.E.; Green, B.N.; Smith, S.
    Cloning, sequencing, and characterization of Escherichia coli thioesterase II (1991), J. Biol. Chem., 266, 11044-11050.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 gene tes B, DNA sequence determination and analysis, no sequence similarity between Escherichia coli thioesterase II and the two types of mammalian thioesterases, i.e. the chain-terminating enzymes of de novo fatty acid synthesis Escherichia coli
3.1.2.2 gene tesB, overexpression in the Escherichia coli host strain Escherichia coli
3.1.2.20 gene tes B, DNA sequence determination and analysis, no sequence similarity between Escherichia coli thioesterase II and the two types of mammalian thioesterases, i.e. the chain-terminating enzymes of de novo fatty acid synthesis Escherichia coli
3.1.2.20 gene tesB, overexpression in the Escherichia coli host strain Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.2 hanging drop method, protein solution: 10 mg/ml, 5 mM NH4OH, 2 mM DTT, over equal volume of reservoir solution: 1-2 M NaCl, 0.1 M sodium formate, pH 6.0, 2 mM N,N-dimethyl-dodecylamine oxide-LDAO, few days, X-ray structure determination and analysis Escherichia coli
3.1.2.20 hanging drop method, protein solution: 10 mg/ml, 5 mM NH4OH, 2 mM DTT, over equal volume of reservoir solution: 1-2 M NaCl, 0.1 M sodium formate, pH 6.0, 2 mM N,N-dimethyl-dodecylamine oxide-LDAO, few days, X-ray structure determination and analysis Escherichia coli

General Stability

EC Number General Stability Organism
3.1.2.2 bovine serum albumin stabilizes Escherichia coli
3.1.2.20 bovine serum albumin stabilizes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 diethyldicarbonate hydroxylamine reverses Escherichia coli
3.1.2.2 IAA
-
 Escherichia coli
3.1.2.2 additional information no inhibition by iodoacetate Escherichia coli
3.1.2.20 diethyldicarbonate hydroxylamine reverses Escherichia coli
3.1.2.20 IAA
-
 Escherichia coli
3.1.2.20 additional information no inhibition by iodoacetate Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Escherichia coli 5829
-
3.1.2.20 cytosol
-
 Escherichia coli 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 123000
-
 recombinant enzyme, native PAGE Escherichia coli
3.1.2.20 123000
-
 recombinant enzyme, native PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Escherichia coli P0AGG2 gene tesB
-
3.1.2.20 Escherichia coli P0AGG2 gene tesB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 native wild-type: 8000fold to homogeneity Escherichia coli
3.1.2.2 recombinant wild-type: 4fold to homogeneity Escherichia coli
3.1.2.20 native wild-type: 8000fold to homogeneity Escherichia coli
3.1.2.20 recombinant wild-type: 4fold to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 93.8
-
 purified recombinant enzyme Escherichia coli
3.1.2.20 93.8
-
 purified recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O
-
 Escherichia coli CoA + a carboxylate
-
 ?
3.1.2.2 arachidoyl-CoA + H2O
-
 Escherichia coli CoA + arachidate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Escherichia coli CoA + decanoate
-
 ?
3.1.2.2 additional information decanoyl pantetheine is no substrate Escherichia coli ?
-
 ?
3.1.2.20 acyl-CoA + H2O
-
 Escherichia coli CoA + a carboxylate
-
 ?
3.1.2.20 arachidoyl-CoA + H2O
-
 Escherichia coli CoA + arachidate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Escherichia coli CoA + decanoate
-
 ?
3.1.2.20 additional information decanoyl pantetheine is no substrate Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 tetramer 4 * 31842-32100, recombinant enzyme, electrospray ionization mass spectroscopy and SDS-PAGE Escherichia coli
3.1.2.20 tetramer 4 * 31842-32100, recombinant enzyme, electrospray ionization mass spectroscopy and SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 25
-
 assay at Escherichia coli
3.1.2.20 25
-
 assay at Escherichia coli