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Literature summary extracted from

  • Murphy, D.J.; Mukherjee, K.D.; Latzko, E.; Woodrow, I.E.
    Solubilization, purification and kinetic properties of three membrane-bound long-chain acyl-coenzyme-A thioesterases from microsomes of photosynthetic tissue (1984), Eur. J. Biochem., 142, 43-48.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 Ca2+
-
 Pisum sativum
3.1.2.20 Ca2+
-
 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 chloroplast
-
 Pisum sativum 9507
-
3.1.2.2 microsome
-
 Pisum sativum
-
-
3.1.2.2 outer membrane
-
 Pisum sativum 19867
-
3.1.2.20 chloroplast
-
 Pisum sativum 9507
-
3.1.2.20 microsome
-
 Pisum sativum
-
-
3.1.2.20 outer membrane
-
 Pisum sativum 19867
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 28000
-
 gel filtration, thioesterase I Pisum sativum
3.1.2.2 139000
-
 gel filtration, thioesterase III Pisum sativum
3.1.2.2 140000
-
 gel filtration, thioesterase II Pisum sativum
3.1.2.20 28000
-
 gel filtration, thioesterase I Pisum sativum
3.1.2.20 139000
-
 gel filtration, thioesterase III Pisum sativum
3.1.2.20 140000
-
 gel filtration, thioesterase II Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Pisum sativum
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Pisum sativum
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Pisum sativum
-
-
-
3.1.2.20 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 100fold, thioesterase II Pisum sativum
3.1.2.2 280fold, thioesterase III Pisum sativum
3.1.2.2 4600fold, thioesterase I Pisum sativum
3.1.2.20 100fold, thioesterase II Pisum sativum
3.1.2.20 280fold, thioesterase III Pisum sativum
3.1.2.20 4600fold, thioesterase I Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 leaf
-
 Pisum sativum
-
3.1.2.20 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 additional information
-
-
 Pisum sativum
3.1.2.20 additional information
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Pisum sativum CoA + hexadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Pisum sativum CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Pisum sativum CoA + oleate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Pisum sativum CoA + palmitate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Pisum sativum CoA + hexadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Pisum sativum CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Pisum sativum CoA + oleate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Pisum sativum CoA + palmitate
-
 ?