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Literature summary extracted from

  • Boyce, S.G.; Luecking, D.R.
    Purification and characterization of long-chain acyl coenzyme A thioesterase from Rhodopseudomonas sphaeroides (1984), Biochemistry, 23, 141-147.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.1.2.2 glycerol and palmitoyl-CoA stabilize Cereibacter sphaeroides
3.1.2.20 glycerol and palmitoyl-CoA stabilize Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 diisopropylfluorophosphate 0.01 mM inhibits Cereibacter sphaeroides
3.1.2.20 diisopropylfluorophosphate 0.01 mM inhibits Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.0000158
-
 hexadecanoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.00084
-
 vaccenoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.0014
-
 linoleoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.00162
-
 oleoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.00186
-
 hexadecanoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.0046
-
 myristoleoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.00786
-
 linolenoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.00867
-
 tetradecanoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.2 0.04468
-
 dodecanoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.0000158
-
 hexadecanoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.00084
-
 vaccenoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.0014
-
 linoleoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.00162
-
 oleoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.00186
-
 hexadecanoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.0046
-
 myristoleoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.00786
-
 linolenoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.00867
-
 tetradecanoyl-CoA
-
 Cereibacter sphaeroides
3.1.2.20 0.04468
-
 dodecanoyl-CoA
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 12500
-
 2 * 12500, SDS-PAGE Cereibacter sphaeroides
3.1.2.2 22400
-
 gel filtration, thioesterase I Cereibacter sphaeroides
3.1.2.2 24000
-
 gel filtration, sedimentation velocity Cereibacter sphaeroides
3.1.2.20 12500
-
 2 * 12500, SDS-PAGE Cereibacter sphaeroides
3.1.2.20 22400
-
 gel filtration, thioesterase I Cereibacter sphaeroides
3.1.2.20 24000
-
 gel filtration, sedimentation velocity Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Cereibacter sphaeroides
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Cereibacter sphaeroides
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Cereibacter sphaeroides
-
-
-
3.1.2.20 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 10000fold Cereibacter sphaeroides
3.1.2.20 10000fold Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 67.5
-
-
 Cereibacter sphaeroides
3.1.2.20 67.5
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C over 1 month Cereibacter sphaeroides
3.1.2.20 -20°C over 1 month Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Cereibacter sphaeroides CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.2 linolenoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + linolenoate
-
 ?
3.1.2.2 linoleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + linoleate
-
 ?
3.1.2.2 myristoleoyl-CoA + H2O
-
 Cereibacter sphaeroides myristoleoate + CoA
-
 ?
3.1.2.2 myristoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + myristate
-
 ?
3.1.2.2 n-butyryl-CoA + H2O
-
 Cereibacter sphaeroides CoA + n-butanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Cereibacter sphaeroides CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + oleate
-
 ?
3.1.2.2 palmitoleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + palmitoleoate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + palmitate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Cereibacter sphaeroides CoA + tetradecanoate
-
 ?
3.1.2.2 vaccenoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + vaccenoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Cereibacter sphaeroides CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
3.1.2.20 linolenoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + linolenoate
-
 ?
3.1.2.20 linoleoyl-CoA
-
 Cereibacter sphaeroides CoA + linoleate
-
 ?
3.1.2.20 myristoleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + myristoleic acid
-
 ?
3.1.2.20 myristoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + myristate
-
 ?
3.1.2.20 n-butyryl-CoA + H2O
-
 Cereibacter sphaeroides CoA + n-butanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Cereibacter sphaeroides CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + oleate
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + palmitoleoate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + palmitate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Cereibacter sphaeroides CoA + tetradecanoate
-
 ?
3.1.2.20 vaccenoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + vaccenoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 dimer 2 * 12500, SDS-PAGE Cereibacter sphaeroides
3.1.2.20 dimer 2 * 12500, SDS-PAGE Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8
-
-
 Cereibacter sphaeroides
3.1.2.20 8
-
-
 Cereibacter sphaeroides