Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Akao, T.; Kusaka, T.; Kobashi, K.
    Two esterases released from Mycobacterium smegmatis for the hydrolysis of long chain acyl-CoAs and Tween (1981), J. Biochem., 90, 1661-1669.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 Ca2+
-
 Mycolicibacterium smegmatis
3.1.2.2 diisopropylfluorophosphate 1 mM inhibits activity completely Mycolicibacterium smegmatis
3.1.2.2 Mg2+
-
 Mycolicibacterium smegmatis
3.1.2.2 papain
-
 Mycolicibacterium smegmatis
3.1.2.2 SDS
-
 Mycolicibacterium smegmatis
3.1.2.2 Triton X-100
-
 Mycolicibacterium smegmatis
3.1.2.2 Trypsin
-
 Mycolicibacterium smegmatis
3.1.2.20 Ca2+
-
 Mycolicibacterium smegmatis
3.1.2.20 diisopropylfluorophosphate 1 mM inhibits activity completely Mycolicibacterium smegmatis
3.1.2.20 Mg2+
-
 Mycolicibacterium smegmatis
3.1.2.20 papain
-
 Mycolicibacterium smegmatis
3.1.2.20 SDS
-
 Mycolicibacterium smegmatis
3.1.2.20 Triton X-100
-
 Mycolicibacterium smegmatis
3.1.2.20 Trypsin
-
 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.0004
-
 eicosanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.2 0.005
-
 oleoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.2 0.0062
-
 hexadecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.2 0.0066
-
 tetradecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.2 0.0077
-
 dodecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.2 0.047
-
 hexadecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.0004
-
 eicosanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.005
-
 oleoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.0062
-
 hexadecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.0066
-
 tetradecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.0077
-
 dodecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.047
-
 hexadecanoyl-CoA
-
 Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 40000
-
 gel filtration Mycolicibacterium smegmatis
3.1.2.2 42000
-
 x * 42000, SDS-PAGE Mycolicibacterium smegmatis
3.1.2.20 40000
-
 gel filtration Mycolicibacterium smegmatis
3.1.2.20 42000
-
 x * 42000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Mycolicibacterium smegmatis
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Mycolicibacterium smegmatis
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Mycolicibacterium smegmatis
-
-
-
3.1.2.20 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2
-
 Mycolicibacterium smegmatis
3.1.2.20
-
 Mycolicibacterium smegmatis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 219
-
-
 Mycolicibacterium smegmatis
3.1.2.20 219
-
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Mycolicibacterium smegmatis CoA + a carboxylate
-
 ?
3.1.2.2 arachidonoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + arachidonate
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Mycolicibacterium smegmatis CoA + dodecanoate
-
 ?
3.1.2.2 eicosanoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + eicosanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
3.1.2.2 monoolein + H2O
-
 Mycolicibacterium smegmatis oleate + glycerol
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Mycolicibacterium smegmatis CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + oleate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + palmitate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Mycolicibacterium smegmatis CoA + tetradecanoate
-
 ?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Mycolicibacterium smegmatis CoA + a carboxylate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + arachidonate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Mycolicibacterium smegmatis CoA + dodecanoate
-
 ?
3.1.2.20 eicosanoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + eicosanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
3.1.2.20 monoolein + H2O
-
 Mycolicibacterium smegmatis oleate + glycerol
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Mycolicibacterium smegmatis CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + oleate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + palmitate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Mycolicibacterium smegmatis CoA + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 ? x * 42000, SDS-PAGE Mycolicibacterium smegmatis
3.1.2.20 ? x * 42000, SDS-PAGE Mycolicibacterium smegmatis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.2 37
-
-
 Mycolicibacterium smegmatis
3.1.2.20 37
-
-
 Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8 9
-
 Mycolicibacterium smegmatis
3.1.2.20 8 9
-
 Mycolicibacterium smegmatis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.2.2 additional information
-
-
 Mycolicibacterium smegmatis
3.1.2.20 additional information
-
-
 Mycolicibacterium smegmatis