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Literature summary extracted from

  • Yabusaki, K.K.; Ballou, C.E.
    Long-chain fatty acyl-CoA thioesterase from Mycobacterium smegmatis (1981), Methods Enzymol., 71, 242-246.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.1.2.2 ovalbumin stabilizes Mycolicibacterium smegmatis
3.1.2.20 ovalbumin stabilizes Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 3-O-methylmannose polysaccharide
-
 Mycolicibacterium smegmatis
3.1.2.2 6-O-methylglucose polysaccharide
-
 Mycolicibacterium smegmatis
3.1.2.20 3-O-methylmannose polysaccharide
-
 Mycolicibacterium smegmatis
3.1.2.20 6-O-methylglucose polysaccharide
-
 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.00013 0.006 fatty-acyl-CoA thioester
-
 Mycolicibacterium smegmatis
3.1.2.2 0.005
-
 hexadecanoyl-CoA micellar Mycolicibacterium smegmatis
3.1.2.2 0.009
-
 hexadecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.2 0.077
-
 CoA
-
 Mycolicibacterium smegmatis
3.1.2.2 0.107
-
 CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.00013 0.006 fatty-acyl-CoA thioester
-
 Mycolicibacterium smegmatis
3.1.2.20 0.005
-
 hexadecanoyl-CoA micellar Mycolicibacterium smegmatis
3.1.2.20 0.009
-
 hexadecanoyl-CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.077
-
 CoA
-
 Mycolicibacterium smegmatis
3.1.2.20 0.107
-
 CoA
-
 Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 20000
-
 2 * 20000, gel electrophoresis Mycolicibacterium smegmatis
3.1.2.2 40000
-
 x * 40000, SDS-PAGE Mycolicibacterium smegmatis
3.1.2.20 20000
-
 2 * 20000, gel electrophoresis Mycolicibacterium smegmatis
3.1.2.20 40000
-
 x * 40000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Mycolicibacterium smegmatis
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Mycolicibacterium smegmatis
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Mycolicibacterium smegmatis
-
-
-
3.1.2.20 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 8000fold Mycolicibacterium smegmatis
3.1.2.20 8000fold Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 230
-
-
 Mycolicibacterium smegmatis
3.1.2.20 230
-
-
 Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 4°C in the presence of 10-20% glycerol Mycolicibacterium smegmatis
3.1.2.20 4°C in the presence of 10-20% glycerol Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Mycolicibacterium smegmatis CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O best substrate Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Mycolicibacterium smegmatis CoA + octadecanoate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + palmitate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Mycolicibacterium smegmatis CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O best substrate Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Mycolicibacterium smegmatis CoA + octadecanoate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 ? x * 40000, SDS-PAGE Mycolicibacterium smegmatis
3.1.2.2 dimer 2 * 20000, gel electrophoresis Mycolicibacterium smegmatis
3.1.2.20 ? x * 40000, SDS-PAGE Mycolicibacterium smegmatis
3.1.2.20 dimer 2 * 20000, gel electrophoresis Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8
-
-
 Mycolicibacterium smegmatis
3.1.2.20 8
-
-
 Mycolicibacterium smegmatis