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Literature summary extracted from

  • Barnes jr., E.M.
    Long-chain fatty acyl thioesterases I and II from Escherichia coli (1975), Methods Enzymol., 35, 102-109.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.2.2 bovine serum albumin stabilizes Escherichia coli
3.1.2.20 bovine serum albumin stabilizes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 diisopropylfluorophosphate 1 mM DFP at 40 min at 25°C produces a 95% inhibition at palmityl thioesterase I Escherichia coli
3.1.2.2 PMSF less effective than DFP, produces a 27% loss of activity under similar conditions Escherichia coli
3.1.2.20 diisopropylfluorophosphate 1 mM DFP at 40 min at 25°C produces a 95% inhibition at palmityl thioesterase I Escherichia coli
3.1.2.20 PMSF less effective than DFP, produces a 27% loss of activity under similar conditions Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 20000
-
-
 Escherichia coli
3.1.2.2 20000 23000 gel filtration Escherichia coli
3.1.2.2 90000
-
 gel filtration, palmityl thioesterase II Escherichia coli
3.1.2.20 20000
-
-
 Escherichia coli
3.1.2.20 20000 23000 gel filtration Escherichia coli
3.1.2.20 90000
-
 gel filtration, palmityl thioesterase II Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Escherichia coli
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Escherichia coli
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Escherichia coli
-
-
-
3.1.2.20 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 20fold, palmityl thioesterase II, palmityl thioesterase I Escherichia coli
3.1.2.2 260fold Escherichia coli
3.1.2.20 20fold, palmityl thioesterase II, palmityl thioesterase I Escherichia coli
3.1.2.20 260fold Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 3.7
-
-
 Escherichia coli
3.1.2.20 3.7
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C over 1 month Escherichia coli
3.1.2.20 -20°C over 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Escherichia coli CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Escherichia coli CoA + hexadecanoate
-
 ir
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Escherichia coli CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Escherichia coli CoA + oleate
-
 ?
3.1.2.2 palmitoleoyl-CoA + H2O
-
 Escherichia coli CoA + palmitoleoate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Escherichia coli CoA + palmitate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Escherichia coli CoA + tetradecanoate
-
 ?
3.1.2.2 vaccenoyl-CoA + H2O cis-isomer Escherichia coli CoA + vaccenoate
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Escherichia coli CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Escherichia coli CoA + hexadecanoate
-
 ir
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Escherichia coli CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Escherichia coli CoA + oleate
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Escherichia coli CoA + palmitoleoate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Escherichia coli CoA + palmitate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Escherichia coli CoA + tetradecanoate
-
 ?
3.1.2.20 vaccenoyl-CoA + H2O cis-isomer Escherichia coli CoA + vaccenoate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8.5
-
-
 Escherichia coli
3.1.2.20 8.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 7 8.5
-
 Escherichia coli
3.1.2.20 7 8.5
-
 Escherichia coli