EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.2 | additional information | cold acclimation induces enzyme activity | Rattus norvegicus | |
3.1.2.18 | additional information | cold acclimation induces enzyme activity | Rattus norvegicus | |
3.1.2.19 | additional information | cold acclimation induces enzyme activity | Rattus norvegicus | |
3.1.2.20 | additional information | cold acclimation induces enzyme activity | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.2 | NADH | no inhibition | Rattus norvegicus | |
3.1.2.18 | beta-NADH | 91% inhibition at 0.4 mM; regulatory function | Rattus norvegicus | |
3.1.2.19 | beta-NADH | weak inhibition, 46% at 0.4 mM | Rattus norvegicus | |
3.1.2.20 | NADH | no inhibition | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.2.2 | cytosol | - |
Rattus norvegicus | 5829 | - |
3.1.2.2 | mitochondrion | matrix | Rattus norvegicus | 5739 | - |
3.1.2.18 | mitochondrial matrix | - |
Rattus norvegicus | 5759 | - |
3.1.2.18 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
3.1.2.18 | additional information | submitochondrial and subcellular distribution | Rattus norvegicus | - |
- |
3.1.2.19 | mitochondrial matrix | - |
Rattus norvegicus | 5759 | - |
3.1.2.19 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
3.1.2.19 | additional information | submitochondrial and subcellular distribution | Rattus norvegicus | - |
- |
3.1.2.20 | cytosol | - |
Rattus norvegicus | 5829 | - |
3.1.2.20 | mitochondrion | matrix | Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 100000 | - |
gel filtration | Rattus norvegicus |
3.1.2.2 | 100000 | - |
below | Rattus norvegicus |
3.1.2.18 | 240000 | - |
at least, gel filtration | Rattus norvegicus |
3.1.2.19 | 100000 | - |
about, gel filtration | Rattus norvegicus |
3.1.2.20 | 100000 | - |
gel filtration | Rattus norvegicus |
3.1.2.20 | 100000 | - |
below | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.18 | acyl-CoA + H2O | Rattus norvegicus | - |
CoA + a carboxylate | - |
? | |
3.1.2.18 | propionyl-CoA + H2O | Rattus norvegicus | ensures that the propionyl-CoA level in mitochondria is not detrimentally increased | CoA + propionate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.2 | Rattus norvegicus | - |
- |
- |
3.1.2.18 | Rattus norvegicus | - |
adult femal Sprague-Dawley rats | - |
3.1.2.19 | Rattus norvegicus | - |
adult female Sprague-Dawley rats | - |
3.1.2.20 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.2 | partial | Rattus norvegicus |
3.1.2.18 | partial | Rattus norvegicus |
3.1.2.19 | partial | Rattus norvegicus |
3.1.2.20 | partial | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.2.2 | brown adipose tissue | - |
Rattus norvegicus | - |
3.1.2.18 | brown adipose tissue | - |
Rattus norvegicus | - |
3.1.2.19 | brown adipose tissue | - |
Rattus norvegicus | - |
3.1.2.20 | brown adipose tissue | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 0.003 | 0.009 | partially purified enzyme, medium- and long chain fatty acid hydrolase activity | Rattus norvegicus |
3.1.2.20 | 0.003 | 0.009 | partially purified enzyme, medium- and long chain fatty acid hydrolase activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.2 | acyl-CoA + H2O | broad specificity for medium-chain to long-chain acyl-CoA | Rattus norvegicus | CoA + a carboxylate | - |
? | |
3.1.2.18 | acetyl-CoA + H2O | low activity | Rattus norvegicus | acetate + CoA | - |
? | |
3.1.2.18 | acyl-CoA + H2O | - |
Rattus norvegicus | CoA + a carboxylate | - |
? | |
3.1.2.18 | propionyl-CoA + H2O | best substrate | Rattus norvegicus | CoA + propionate | - |
? | |
3.1.2.18 | propionyl-CoA + H2O | ensures that the propionyl-CoA level in mitochondria is not detrimentally increased | Rattus norvegicus | CoA + propionate | - |
? | |
3.1.2.19 | additional information | broad substrate spectrum, substrate specificity | Rattus norvegicus | ? | - |
? | |
3.1.2.19 | additional information | from the substrate specificity curves it is not possible to conclude whether the long-chain acyl-CoA hydrolase activity is due to the broad-spectrum enzyme or to a different long-chain acyl-CoA hydrolase | Rattus norvegicus | ? | - |
? | |
3.1.2.19 | nonanoyl-CoA + H2O | best substrate | Rattus norvegicus | CoA + nonanoate | - |
? | |
3.1.2.20 | acyl-CoA + H2O | broad specificity for medium-chain to long-chain acyl-CoA | Rattus norvegicus | CoA + a carboxylate | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 7.4 | - |
assay at | Rattus norvegicus |
3.1.2.18 | 7.4 | - |
assay at | Rattus norvegicus |
3.1.2.20 | 7.4 | - |
assay at | Rattus norvegicus |