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Literature summary extracted from

  • Anand, G.S.; Stock, A.M.
    Kinetic basis for the stimulatory effect of phosphorylation on the methylesterase activity of CheB (2002), Biochemistry, 41, 6752-6760.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.61 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 expression in Escherichia coli Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.61 additional information constructs of the N- and C-terminal domains and investigation of interaction between the isolated regulatory and effector domains of enzyme Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 additional information constructs of the N- and C-terminal domains and investigation of interaction between the isolated regulatory and effector domains of enzyme Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.61 additional information the unphosphorylated regulator domain partially inhibits methylesterase Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 additional information the unphosphorylated regulator domain partially inhibits methylesterase Thermotoga maritima
3.1.1.61 P2 domain of histidine kinase inhibition is decreased upon phosphorylation Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.61 additional information
-
 additional information
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.61 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
3.1.1.61 Thermotoga maritima
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.61 phosphoprotein In its unphosphorylated state, the regulatory domain inhibits enzyme activity of effector domain. Phosphorylation of the regulator domain leads to an enhancement of enzyme activity through a relief of inhibition and a stimulatory effect on catalysis. Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 phosphoprotein In its unphosphorylated state, the regulatory domain inhibits enzyme activity of effector domain. Phosphorylation of the regulator domain leads to an enhancement of enzyme activity through a relief of inhibition and a stimulatory effect on catalysis. Thermotoga maritima

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.61 recombinant enzyme Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 recombinant enzyme Thermotoga maritima

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.61 protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol the enzyme is a response regulator protein in the bacterial chemotaxis two-component signal transduction pathway Salmonella enterica subsp. enterica serovar Typhimurium
a

Subunits

EC Number Subunits Comment Organism
3.1.1.61 More the CheB protein is composed of at least 2 structurally distinct proportions: a C-terminal catalytic domain, and a N-terminal region which modulates esterase activity Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 More the CheB protein is composed of at least 2 structurally distinct proportions: a C-terminal catalytic domain, and a N-terminal region which modulates esterase activity Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.61 additional information
-
 additional information
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.61 0.0025
-
 P2 domain of histidine kinase pH 7.0, 25°C, in absence of phosphoramidate Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 0.021
-
 P2 domain of histidine kinase pH 7.0, 25°C, in presence of phosphoramidate Salmonella enterica subsp. enterica serovar Typhimurium