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Literature summary extracted from

  • Snyder, M.A.; Stock, J.B.; Koshland, D.E.
    Carboxylmethyl esterase of bacterial chemotaxis (1984), Methods Enzymol., 106, 321-330.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.61 maximal stability and low activity is observed in phosphate buffer at pH 7 with 50-100 mM added NaCl, while MES and low salt concentration afford minimum protection against inactivation Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.61 2,4,6-Trinitrobenzene sulfonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 2-Hydroxy-5-nitrobenzyl bromide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 5,5'-dithiobis(2-nitrobenzoate)
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 diethyl dicarbonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 iodoacetamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 iodoacetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 methyl methanothiosulfonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 N-Acetylimidazole
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 NEM slight Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 Phenylglyoxal
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.61 0.015
-
 methyl-accepting chemotaxis protein pH 7.0, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 0.026
-
 methylated prolactin pH 7.0, 30°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.61 Ca2+ as effective as Mg2+ in activation Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.61 Mg2+ divalent cation required Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.61 37000
-
 x * 37000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.61 additional information Salmonella enterica subsp. enterica serovar Typhimurium responsible for the demethylation of the methyl-accepting chemotaxis proteins ?
-
 ?
3.1.1.61 additional information Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 responsible for the demethylation of the methyl-accepting chemotaxis proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.61 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
3.1.1.61 Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.61 strain ST89pGK2, plasmid bearing strain overproducing esterase and transferase Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.61 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
3.1.1.61 4°C, 10% glycerol, 0.04% sodium azide, stable for at least several months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.61 methyl-accepting chemotaxis protein + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium chemotaxis protein + methanol
-
 ?
3.1.1.61 methyl-accepting chemotaxis protein + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 chemotaxis protein + methanol
-
 ?
3.1.1.61 methylated prolactin + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium prolactin + methanol
-
 ?
3.1.1.61 methylated prolactin + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 prolactin + methanol
-
 ?
3.1.1.61 additional information responsible for the demethylation of the methyl-accepting chemotaxis proteins Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.1.1.61 additional information responsible for the demethylation of the methyl-accepting chemotaxis proteins Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.61 ? x * 37000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.61 0.004
-
 methyl-accepting chemotaxis protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.61 5.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.61 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
 9.3