EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.3.6 | 24200 | - |
alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE | Pseudomonas putida |
2.8.3.6 | 25300 | - |
alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE | Pseudomonas putida |
2.8.3.6 | 25600 | - |
alpha2beta2, 2 * 25600 + 2 * 26500, SDS-PAGE | Acinetobacter calcoaceticus |
2.8.3.6 | 26500 | - |
alpha2beta2, 2 * 25600 + 2 * 26500, SDS-PAGE | Acinetobacter calcoaceticus |
2.8.3.6 | 108000 | - |
gel filtration | Acinetobacter calcoaceticus |
2.8.3.6 | 109000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.6 | succinyl-CoA + 3-oxoadipate | Pseudomonas putida | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | ? | - |
? | |
2.8.3.6 | succinyl-CoA + 3-oxoadipate | Acinetobacter calcoaceticus | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.6 | Acinetobacter calcoaceticus | - |
enzymes from Pseudomonas putida and Acinetobacter are immunologically related, not identical | - |
2.8.3.6 | Pseudomonas putida | - |
enzymes from Pseudomonas putida and Acinetobacter are immunologically related, not identical | - |
2.8.3.6 | Pseudomonas putida | - |
strains PRS 2260 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.3.6 | - |
Pseudomonas putida |
2.8.3.6 | transferase I | Acinetobacter calcoaceticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.3.6 | 21.3 | - |
- |
Pseudomonas putida |
2.8.3.6 | 24.2 | - |
transferase I | Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.6 | succinyl-CoA + 3-oxoadipate | - |
Pseudomonas putida | succinate + 3-oxoadipyl-CoA | - |
? | |
2.8.3.6 | succinyl-CoA + 3-oxoadipate | - |
Acinetobacter calcoaceticus | succinate + 3-oxoadipyl-CoA | - |
? | |
2.8.3.6 | succinyl-CoA + 3-oxoadipate | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | Pseudomonas putida | ? | - |
? | |
2.8.3.6 | succinyl-CoA + 3-oxoadipate | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | Acinetobacter calcoaceticus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.3.6 | More | amino acid composition | Pseudomonas putida |
2.8.3.6 | More | amino acid composition | Acinetobacter calcoaceticus |
2.8.3.6 | tetramer | alpha2beta2, 2 * 25600 + 2 * 26500, SDS-PAGE | Acinetobacter calcoaceticus |
2.8.3.6 | tetramer | alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.6 | 40 | - |
t1/2: 26 min | Pseudomonas putida |
2.8.3.6 | 40 | 45 | 30 min, stable | Acinetobacter calcoaceticus |
2.8.3.6 | 45 | - |
t1/2: 4 min | Pseudomonas putida |
2.8.3.6 | 50 | - |
t1/2: 1 min | Pseudomonas putida |
2.8.3.6 | 50 | - |
30 min, 10% loss of activity | Acinetobacter calcoaceticus |