EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.3.16 | DNA sequence determination and analysis, monocistronic operon, overexpression in Escherichia coli, activity is similar to the wild-type enzyme | Oxalobacter formigenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.3.16 | 45000 | - |
1 * 45000, recombinant enzyme, SDS-PAGE | Oxalobacter formigenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.16 | formyl-CoA + oxalate | Oxalobacter formigenes | activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora | formate + oxalyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.16 | Oxalobacter formigenes | O06644 | gene frc | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.3.16 | recombinant from Escherichia coli JM109 | Oxalobacter formigenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.16 | formyl-CoA + oxalate | - |
Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
2.8.3.16 | formyl-CoA + oxalate | activation-decarboxylation reaction in the catabolism of oxalic acid, degradation and detoxification in mammalian intestinal flora | Oxalobacter formigenes | formate + oxalyl-CoA | - |
? | |
2.8.3.16 | succinyl-CoA + oxalate | no activity with the recombinant enzyme expressed in Escherichia coli | Oxalobacter formigenes | succinate + oxalyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.3.16 | monomer | 1 * 45000, recombinant enzyme, SDS-PAGE | Oxalobacter formigenes |