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Literature summary extracted from

  • Buckel, W.; Dorn, U.; Semmler, R.
    Glutaconate CoA-transferase from Acidaminococcus fermentans (1981), Eur. J. Biochem., 118, 315-321.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.3.12
-
Acidaminococcus fermentans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.12 4-hydroxymercuribenzoate 0.2 mM, at 37°C, 2 min, 80% loss of activity, 2-mercaptoethanol partially restores activity Acidaminococcus fermentans
2.8.3.12 additional information not inhibited by 2 mM iodoacetate, 2 mM iodoacetamide or 1 mM 5,5’-dithiobis(2-nitrobenzoate) Acidaminococcus fermentans
2.8.3.12 NaBH4 10 mM, in presence of 0.4 mM glutyral-CoA, at 25°C, 30 min, almost complete inhibition Acidaminococcus fermentans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.12 0.015
-
glutaryl-CoA pH 7, 25°C, cosubstrate acetate Acidaminococcus fermentans
2.8.3.12 0.017
-
glutaconyl-CoA cosubstrate glutarate Acidaminococcus fermentans
2.8.3.12 0.17
-
acetyl-CoA cosubstrate glutaconate Acidaminococcus fermentans
2.8.3.12 0.2
-
glutaconate pH 7, 25°C, cosubstrate acetyl-CoA Acidaminococcus fermentans
2.8.3.12 0.7
-
Glutarate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 1.5
-
(R)-2-hydroxyglutarate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 8
-
adipate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 10
-
Acrylate cosubstrate acetyl-CoA Acidaminococcus fermentans
2.8.3.12 13
-
3-hydroxyglutarate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 14
-
(S)-2-hydroxyglutarate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 16
-
propionate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 26
-
acetate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 100
-
isocrotonate cosubstrate acetyl-CoA Acidaminococcus fermentans
2.8.3.12 150
-
Butyrate cosubstrate glutaconyl-CoA Acidaminococcus fermentans
2.8.3.12 500
-
crotonate cosubstrate acetyl-CoA Acidaminococcus fermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.12 32000
-
alpha4beta4, 4 * 32000 + 4 * 34000, SDS-PAGE Acidaminococcus fermentans
2.8.3.12 34000
-
alpha4beta4, 4 * 32000 + 4 * 34000, SDS-PAGE Acidaminococcus fermentans
2.8.3.12 275000
-
gel filtration Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.12 acetyl-CoA + (E)-glutaconate Acidaminococcus fermentans involved in glutamate fermentation via hydroxyglutarate pathway, activates glutaconate to glutaconyl-CoA prior to glutaconate decarboxylation acetate + glutaconyl-1-CoA
-
?
2.8.3.12 additional information Acidaminococcus fermentans not involved in the dehydration of (R)-2-hydroxyglutarate to glutaconate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.12 Acidaminococcus fermentans
-
ATCC 25085
-
2.8.3.12 no activity in Clostridium tetanomorphum
-
-
-
2.8.3.12 no activity in Clostridium tetanomorphum H1
-
-
-
2.8.3.12 [Clostridium] sporosphaeroides
-
NCIB 10672
-
2.8.3.12 [Clostridium] symbiosum
-
HB 25
-
2.8.3.12 [Clostridium] symbiosum HB 25
-
HB 25
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.12
-
Acidaminococcus fermentans

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.3.12 acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA ping-pong mechanism Acidaminococcus fermentans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.12 additional information
-
-
[Clostridium] sporosphaeroides
2.8.3.12 additional information
-
-
[Clostridium] symbiosum
2.8.3.12 additional information
-
-
Acidaminococcus fermentans

Storage Stability

EC Number Storage Stability Organism
2.8.3.12 4°C, crystalline suspension in ammonium sulfate, 2 years, stable Acidaminococcus fermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.12 acetyl-CoA + (E)-glutaconate
-
[Clostridium] sporosphaeroides acetate + glutaconyl-1-CoA
-
?
2.8.3.12 acetyl-CoA + (E)-glutaconate
-
[Clostridium] symbiosum acetate + glutaconyl-1-CoA
-
?
2.8.3.12 acetyl-CoA + (E)-glutaconate very good substrate, broad substrate specificity Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
?
2.8.3.12 acetyl-CoA + (E)-glutaconate very good substrate, broad substrate specificity Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
r
2.8.3.12 acetyl-CoA + (E)-glutaconate removal of 3'-phospho group from acetyl-CoA leads to reduced activity Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
?
2.8.3.12 acetyl-CoA + (E)-glutaconate removal of 3'-phospho group from acetyl-CoA leads to reduced activity Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
r
2.8.3.12 acetyl-CoA + (E)-glutaconate involved in glutamate fermentation via hydroxyglutarate pathway, activates glutaconate to glutaconyl-CoA prior to glutaconate decarboxylation Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
?
2.8.3.12 acetyl-CoA + (E)-glutaconate
-
[Clostridium] symbiosum HB 25 acetate + glutaconyl-1-CoA
-
?
2.8.3.12 acetyl-CoA + (R)-2-hydroxyglutarate
-
Acidaminococcus fermentans acetate + (R)-2-hydroxyglutaryl-1-CoA in vitro formation of both isomers: (R)-2-hydroxyglutaryl-1-CoA and (R)-2-hydroxyglutaryl-5-CoA, the 1-isomer is kinetically favoured, the 5-isomer is thermodynamically favoured ?
2.8.3.12 acetyl-CoA + acrylate poor substrate Acidaminococcus fermentans acrylyl-CoA + acetate
-
?
2.8.3.12 acetyl-CoA + crotonate poor substrate Acidaminococcus fermentans crotonyl-CoA + acetate
-
?
2.8.3.12 acetyl-CoA + isocrotonate poor substrate Acidaminococcus fermentans isocrotonyl-CoA + acetate
-
?
2.8.3.12 glutaconyl-CoA + (R)-2-hydroxyglutarate very good substrate Acidaminococcus fermentans glutaconate + (R)-2-hydroxyglutaryl-CoA generation of both isomers: (R)-2-hydroxyglutaryl-1-CoA and (R)-2-hydroxyglutaryl-5-CoA ?
2.8.3.12 glutaconyl-CoA + (S)-2-hydroxyglutarate at 60% of the rate with (R)-isomer or glutarate Acidaminococcus fermentans glutaconate + (S)-2-hydroxyglutaryl-CoA
-
?
2.8.3.12 glutaconyl-CoA + 3-hydroxyglutarate at 20% of the rate with glutarate Acidaminococcus fermentans glutaconate + 3-hydroxyglutaryl-CoA
-
?
2.8.3.12 glutaconyl-CoA + adipate at 60% of the rate with glutarate Acidaminococcus fermentans glutaconate + adipyl-CoA
-
?
2.8.3.12 glutaconyl-CoA + butyrate
-
Acidaminococcus fermentans butyryl-CoA + glutaconate
-
?
2.8.3.12 glutaconyl-CoA + glutarate very good substrate Acidaminococcus fermentans glutaconate + glutaryl-CoA
-
?
2.8.3.12 glutaconyl-CoA + propionate moderate substrate Acidaminococcus fermentans propanoyl-CoA + glutaconate
-
?
2.8.3.12 glutaryl-CoA + acetate
-
Acidaminococcus fermentans glutarate + acetyl-CoA
-
r
2.8.3.12 additional information no substrates: (Z)-glutaconate, acetyl-4'-phosphopantetheine, C4-dicarboxylic acids, reverse reaction not with glutamate, 2-oxoglutarate, succinate, malate or citrate Acidaminococcus fermentans ?
-
?
2.8.3.12 additional information not involved in the dehydration of (R)-2-hydroxyglutarate to glutaconate Acidaminococcus fermentans ?
-
?

Subunits

EC Number Subunits Comment Organism
2.8.3.12 octamer alpha4beta4, 4 * 32000 + 4 * 34000, SDS-PAGE Acidaminococcus fermentans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.12 25
-
assay at [Clostridium] sporosphaeroides
2.8.3.12 25
-
assay at [Clostridium] symbiosum
2.8.3.12 25
-
assay at Acidaminococcus fermentans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.12 7
-
assay at Acidaminococcus fermentans
2.8.3.12 7
-
optimum, glutaconyl-CoA + acetate Acidaminococcus fermentans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.3.12 5.6 7.4 about 70% of maximal activity at pH 5.6 and 7.4 Acidaminococcus fermentans
2.8.3.12 8.5
-
about 10% of maximal activity at pH 8.5 Acidaminococcus fermentans