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Literature summary extracted from

  • Tomizuka, T.; Oeda, T.; Tamura, Y.; Yoshida, S.; Strott, C.A.
    Characterization of guinea pig estrogen sulfotransferase expressed by Chinese hamster ovary cell-K1 stable transfectants (1994), Endocrinology, 135, 938-943.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.4 EST cloned and expressed by CHO-K1 cells stably transfected with plasmid pcDNAI/Neo eukaryotic expression vector Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.4 3'-phosphoadenylylsulfate competitive inhibition Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.4 0.00004
-
 estriol pH 7.4, 37°C Cavia porcellus
2.8.2.4 0.00006
-
 estrone pH 7.4, 37°C Cavia porcellus
2.8.2.4 0.00007
-
 17beta-estradiol pH 7.4, 37°C Cavia porcellus
2.8.2.4 0.0012
-
 3'-phosphoadenylylsulfate pH 7.4, 37°C Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.4 cytosol
-
 Cavia porcellus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.4 34000
-
 native and recombinant enzyme, immunoblot analysis Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.4 Cavia porcellus
-
 guinea pig
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.4 3'-phosphoadenylylsulfate + estriol
-
 Cavia porcellus adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
 ?
2.8.2.4 3'-phosphoadenylylsulfate + estrone
-
 Cavia porcellus adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.4 0.00052
-
 3'-phosphoadenylylsulfate pH 7.4, 37°C, competitive inhibition Cavia porcellus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.4 Cavia porcellus isoelectric focusing, multiple charge isoforms
-
 5.2
2.8.2.4 Cavia porcellus isoelectric focusing, multiple charge isoforms
-
 5.4
2.8.2.4 Cavia porcellus isoelectric focusing, multiple charge isoforms
-
 5.5
2.8.2.4 Cavia porcellus isoelectric focusing, multiple charge isoforms
-
 6.4