EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.4 | monothiolglycerol | activity is stimulated 10fold by 25 mM | Cavia porcellus |
EC Number | General Stability | Organism |
---|---|---|
2.8.2.4 | not stabilized by 20% glycerol or 25 mM NaCl | Cavia porcellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.4 | ZnCl2 | inhibition of liver isoenzyme at 15 mM | Cavia porcellus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.8.2.4 | cytosol | - |
Cavia porcellus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.4 | Ca2+ | activity is stimulated 2fold by 15 mM CaCl2 | Cavia porcellus | |
2.8.2.4 | Mg2+ | activity is stimulated 2fold by 15 mM MgCl2 | Cavia porcellus | |
2.8.2.4 | Zn2+ | weakly stimulatory at 2.5 mM ZnCl2 | Cavia porcellus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.2.4 | 50000 | - |
chorion enzyme, gel filtration | Cavia porcellus |
2.8.2.4 | 52300 | - |
liver enzyme, gel filtration | Cavia porcellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.4 | 3'-phosphoadenylylsulfate + estradiol | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
2.8.2.4 | 3'-phosphoadenylylsulfate + estrone | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.4 | Cavia porcellus | - |
guinea pig | - |
2.8.2.4 | Cavia porcellus | - |
english shorthair | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.4 | partial, liver and chorion isoenzymes | Cavia porcellus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.2.4 | chorion | - |
Cavia porcellus | - |
2.8.2.4 | liver | - |
Cavia porcellus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.2.4 | -20°C, 25 mM monothiolglycerol, 20% loss of activity in 1 week | Cavia porcellus |
2.8.2.4 | -20°C, partially purified enzyme exhibits large losses of activity within 1 week | Cavia porcellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.4 | 3'-phosphoadenylylsulfate + estradiol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
2.8.2.4 | 3'-phosphoadenylylsulfate + estrone | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
2.8.2.4 | additional information | activity toward dehydroepiandrosterone and testosterone is minimal or undetectable | Cavia porcellus | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.4 | 8 | - |
liver isoenzyme | Cavia porcellus |