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Literature summary extracted from

  • Ramaswamy, S.G.; Jakoby, W.B.
    Amine N-sulfotransferase (1987), J. Biol. Chem., 262, 10039-10043.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.3 additional information activity is dependent on the presence of unprotonated amino groups Cavia porcellus
2.8.2.3 additional information activity is dependent on the presence of unprotonated amino groups Rattus norvegicus
2.8.2.3 additional information activity is dependent on the presence of unprotonated amino groups Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.3 0.03
-
 3'-phosphoadenylylsulfate with 2-naphthylamine as second substrate, pH 8, 37°C Cavia porcellus
2.8.2.3 0.03
-
 3'-phosphoadenylylsulfate with 2-naphthylamine as second substrate, pH 8, 37°C Rattus norvegicus
2.8.2.3 0.03
-
 3'-phosphoadenylylsulfate with 2-naphthylamine as second substrate, pH 8, 37°C Oryctolagus cuniculus
2.8.2.3 14
-
 1,2,3,4-tetrahydroisoquinoline pH 8, 37°C Cavia porcellus
2.8.2.3 38
-
 1,2,3,4-tetrahydroquinoline pH 8, 37°C Cavia porcellus
2.8.2.3 3400
-
 2-Naphthylamine pH 8, 37°C Cavia porcellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.3 Mg2+ required Cavia porcellus
2.8.2.3 Mg2+ required Rattus norvegicus
2.8.2.3 Mg2+ required Oryctolagus cuniculus
2.8.2.3 Mg2+ optimal stimulation: 20 mM MgCl2 Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.3 33000
-
 x * 33000 + x * 34000, SDS-PAGE Cavia porcellus
2.8.2.3 34000
-
 x * 33000 + x * 34000, SDS-PAGE Cavia porcellus
2.8.2.3 60000
-
 gel filtration Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.3 3'-phosphoadenylylsulfate + 2-naphthylamine Cavia porcellus
-
 adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
-
 ir
2.8.2.3 3'-phosphoadenylylsulfate + 2-naphthylamine Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + 2-naphthylamine Oryctolagus cuniculus
-
 adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + aniline Cavia porcellus
-
 adenosine 3',5'-bisphosphate + N-sulfoaniline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.3 Cavia porcellus
-
-
-
2.8.2.3 Oryctolagus cuniculus
-
 rabbit
-
2.8.2.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.3
-
 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.3 intestine
-
 Cavia porcellus
-
2.8.2.3 intestine
-
 Rattus norvegicus
-
2.8.2.3 kidney
-
 Rattus norvegicus
-
2.8.2.3 liver
-
 Cavia porcellus
-
2.8.2.3 liver
-
 Rattus norvegicus
-
2.8.2.3 liver
-
 Oryctolagus cuniculus
-
2.8.2.3 mucosa
-
 Cavia porcellus
-
2.8.2.3 mucosa
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.3 0.05
-
-
 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.3 3'-phosphoadenylylsulfate + 1,2,3,4-tetrahydroisoquinoline
-
 Cavia porcellus adenosine 3',5'-bisphosphate + 1,2,3,4-tetrahydroisoquinoline N-sulfate
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + 1,2,3,4-tetrahydroquinoline
-
 Cavia porcellus adenosine 3',5'-bisphosphate + 1,2,3,4-tetrahydroquinoline N-sulfate
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + 2-naphthylamine
-
 Cavia porcellus adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
-
 ir
2.8.2.3 3'-phosphoadenylylsulfate + 2-naphthylamine
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + 2-naphthylamine
-
 Oryctolagus cuniculus adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + aniline
-
 Cavia porcellus adenosine 3',5'-bisphosphate + N-sulfoaniline
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + cyclohexylamine
-
 Cavia porcellus adenosine 3',5'-bisphosphate + N-sulfocyclohexylamine
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + desmethylimipramine
-
 Cavia porcellus adenosine 3',5'-bisphosphate + demethylimipramine N-sulfate
-
 ?
2.8.2.3 3'-phosphoadenylylsulfate + octylamine
-
 Cavia porcellus adenosine 3',5'-bisphosphate + N-sulfooctylamine
-
 ?
2.8.2.3 additional information acceptor: primary amines, secondary amines, purified preparation also has O-sulfotransferase activities, suggesting that transfer to oxygen could represent an intrinsic function of N-sulfotransferase Cavia porcellus ?
-
 ?
2.8.2.3 additional information acceptor: primary amines, secondary amines, purified preparation also has O-sulfotransferase activities, suggesting that transfer to oxygen could represent an intrinsic function of N-sulfotransferase Rattus norvegicus ?
-
 ?
2.8.2.3 additional information acceptor: primary amines, secondary amines, purified preparation also has O-sulfotransferase activities, suggesting that transfer to oxygen could represent an intrinsic function of N-sulfotransferase Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.3 ? x * 33000 + x * 34000, SDS-PAGE Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.3 37
-
 assay at Cavia porcellus
2.8.2.3 37
-
 assay at Rattus norvegicus
2.8.2.3 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.3 6.8
-
 assay at Cavia porcellus
2.8.2.3 6.8
-
 assay at Rattus norvegicus
2.8.2.3 6.8
-
 assay at Oryctolagus cuniculus
2.8.2.3 8
-
 assay at Cavia porcellus
2.8.2.3 8
-
 assay at Rattus norvegicus
2.8.2.3 8
-
 assay at Oryctolagus cuniculus