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Literature summary extracted from

  • Kwon, A.R.; Oh, T.G.; Kim, D.H.; Choi, E.C.
    Molecular cloning of the arylsulfate sulfotransferase gene and characterization of its product from Enterobacter amnigenus AR-37 (1999), Protein Expr. Purif., 17, 366-372.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.22
-
 Lelliottia amnigena

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.22 0.163
-
 4-nitrophenyl sulfate pH 9, 37°C, with phenol as second substrate Lelliottia amnigena
2.8.2.22 0.314
-
 phenol pH 9, 37°C, with 4-nitrophenyl sulfate as second substrate Lelliottia amnigena

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 63880
-
 calculated from nucleotide sequence Lelliottia amnigena
2.8.2.22 64000
-
 SDS-PAGE, gel filtration, wild-type and mutant enzymes Lelliottia amnigena

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Lelliottia amnigena Q9XDP2 AR-37
-
2.8.2.22 Lelliottia amnigena AR-37 Q9XDP2 AR-37
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22 recombinant protein Lelliottia amnigena

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.22 additional information
-
-
 Lelliottia amnigena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 2-naphthyl sulfate + phenol
-
 Lelliottia amnigena 2-naphthol + phenyl sulfate
-
 ?
2.8.2.22 4-methylumbelliferyl sulfate + phenol
-
 Lelliottia amnigena 4-methylumbelliferol + phenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-acetaminophen
-
 Lelliottia amnigena 4-nitrophenol + 4-acetaminophenylsulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-acetaminophen
-
 Lelliottia amnigena AR-37 4-nitrophenol + 4-acetaminophenylsulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + alpha-naphthol best acceptor Lelliottia amnigena 4-nitrophenol + 1-naphththyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + alpha-naphthol best acceptor Lelliottia amnigena AR-37 4-nitrophenol + 1-naphththyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 Lelliottia amnigena phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 Lelliottia amnigena AR-37 phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + resorcinol
-
 Lelliottia amnigena resorcinol O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + resorcinol
-
 Lelliottia amnigena AR-37 resorcinol O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyramine
-
 Lelliottia amnigena 4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyramine
-
 Lelliottia amnigena AR-37 4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyrosine
-
 Lelliottia amnigena tyrosine O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 alpha-naphthyl sulfate + phenol
-
 Lelliottia amnigena alpha-naphthol + phenyl sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 monomer 1 * 64000, SDS-PAGE Lelliottia amnigena

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.22 9
-
-
 Lelliottia amnigena

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.22 Lelliottia amnigena calculated from amino acid sequence, isoelectric focussing
-
 9