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Literature summary extracted from

  • Hanai, H.; Nakayama, D.; Yang, H.; Matsubayashi, Y.; Hirota, Y.; Sakagami, Y.
    Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O-sulfation of preprophytosulfokine variants in vitro (2000), FEBS Lett., 470, 97-101.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.20 0.071
-
 EEFHTDYIYTQDVK PP-PSK-(74-87), truncated sequence of preprophytosulfokinase, pH 7, 30°C Oryza sativa
2.8.2.20 0.457
-
 EEFHTDYIYTQDVK PP-PSK-(74-87), truncated sequence of preprophytosulfokinase, pH 7, 30°C Asparagus officinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 microsome
-
 Asparagus officinalis
-
-
2.8.2.20 microsome
-
 Nicotiana tabacum
-
-
2.8.2.20 microsome
-
 Daucus carota
-
-
2.8.2.20 microsome
-
 Solanum lycopersicum
-
-
2.8.2.20 microsome
-
 Oryza sativa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.20 Mg2+ 1.2fold at 5 mM Asparagus officinalis
2.8.2.20 Mn2+
-
 Asparagus officinalis

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Asparagus officinalis
-
-
-
2.8.2.20 Daucus carota
-
-
-
2.8.2.20 Nicotiana tabacum
-
-
-
2.8.2.20 Oryza sativa
-
-
-
2.8.2.20 Solanum lycopersicum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.20 Oc cell
-
 Oryza sativa
-
2.8.2.20 plant cell line
-
 Asparagus officinalis
-
2.8.2.20 TBY-2 cell
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylylsulfate + DYIYTQDVK PP-PSK-(79-87), truncated sequence of preprophytosulfokinase, poor substrate Asparagus officinalis adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + DYIYTQDVK PP-PSK-(79-87), truncated sequence of preprophytosulfokinase, poor substrate Oryza sativa adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + EEFHTDYIYTQDVK PP-PSK-(74-87), truncated sequence of preprophytosulfokinase Asparagus officinalis adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + EEFHTDYIYTQDVK PP-PSK-(74-87), truncated sequence of preprophytosulfokinase Oryza sativa adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + FHTDYIYTQDVK PP-PSK-(76-87), truncated sequence of preprophytosulfokinase, poor substrate Asparagus officinalis adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + FHTDYIYTQDVK PP-PSK-(76-87), truncated sequence of preprophytosulfokinase, poor substrate Oryza sativa adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK PP-PSK-(73-87), truncated sequence of preprophytosulfokinase Asparagus officinalis adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK PP-PSK-(73-87), truncated sequence of preprophytosulfokinase Nicotiana tabacum adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK PP-PSK-(73-87), truncated sequence of preprophytosulfokinase Daucus carota adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK PP-PSK-(73-87), truncated sequence of preprophytosulfokinase Solanum lycopersicum adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + WEEFHTDYIYTQDVK PP-PSK-(73-87), truncated sequence of preprophytosulfokinase Oryza sativa adenosine 3',5'-bisphosphate + ?
-
 ?