Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kasinathan, C.; Sundaram, P.; Slomiany, B.L.; Slomiany, A.
    Inhibition of tyrosylprotein sulfotransferase by sphingosine and its reversal by acidic phospholipids (1993), Biochemistry, 32, 1194-1198.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.20 lysophosphatidylcholine slight activation, 0.2 mM Rattus norvegicus
2.8.2.20 oleic acid activation, 0.2 mM Rattus norvegicus
2.8.2.20 phosphatidylinositol slight activation, 0.2 mM Rattus norvegicus
2.8.2.20 phosphatidylserine slight activation, 0.2 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.20 D-erythro-sphinganine less effective than sphingosine Rattus norvegicus
2.8.2.20 L-threo-sphinganine
-
 Rattus norvegicus
2.8.2.20 phosphatidylcholine weak Rattus norvegicus
2.8.2.20 psychosine almost complete inhibition Rattus norvegicus
2.8.2.20 sphingomyelin weak Rattus norvegicus
2.8.2.20 sphingosine 50% inhibition at 0.15 mM, kinetics, enhanced by phosphatidylcholine or sphingomyelin. Phosphatidylinositol, phosphatidylserine or oleic acid reverses Rattus norvegicus
2.8.2.20 Stearylamine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.20 0.00004
-
 EAY acidic amino acid polymer, pH 6.8, 37°C Rattus norvegicus
2.8.2.20 0.00025
-
 3'-phosphoadenylylsulfate pH 6.8, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 Golgi membrane
-
 Rattus norvegicus 139
-
2.8.2.20 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.20 Mn2+ requirement Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Rattus norvegicus
-
 male Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.20 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.20 brain
-
 Rattus norvegicus
-
2.8.2.20 cerebellum
-
 Rattus norvegicus
-
2.8.2.20 heart
-
 Rattus norvegicus
-
2.8.2.20 liver
-
 Rattus norvegicus
-
2.8.2.20 lung
-
 Rattus norvegicus
-
2.8.2.20 salivary gland submandibular Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylyl sulfate + EAY
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + EAY(O-sulfate)
-
 ?
2.8.2.20 3'-phosphoadenylylsulfate + acidic amino acid polymer EAY i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.20 37
-
 assay at Rattus norvegicus