EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.2 | 0.012 | - |
dehydroepiandrosterone | - |
Rattus norvegicus | |
2.8.2.2 | 0.035 | - |
beta-estradiol | - |
Rattus norvegicus | |
2.8.2.2 | 0.07 | - |
testosterone | - |
Rattus norvegicus | |
2.8.2.2 | 0.17 | - |
aldosterone | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.29 | - |
cortisol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.35 | - |
estriol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.44 | - |
cortisol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.52 | - |
11-deoxycorticosterone | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 1 | - |
Hexanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 1.2 | - |
3-methyl-1-butanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 1.7 | - |
1-Pentanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 3 | - |
1-butanol | - |
Rattus norvegicus | |
2.8.2.2 | 17 | - |
2-propanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 24 | - |
1-propanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 42 | - |
ethanol | - |
Rattus norvegicus | |
2.8.2.2 | 47 | - |
ethanol | pH 7.5, 37°C | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.2.2 | 28000 | - |
6 * 28000, SDS-PAGE | Rattus norvegicus |
2.8.2.2 | 180000 | - |
gel filtration | Rattus norvegicus |
2.8.2.2 | 180000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.2 | 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.2 | Rattus norvegicus | - |
rat | - |
2.8.2.2 | Rattus norvegicus | - |
Sprague-Dawley, female | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.2 | sulfotransferase 1 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.2.2 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.2.2 | 0.11 | - |
substrate estradiol | Rattus norvegicus |
2.8.2.2 | 0.15 | - |
substrate n-butanol | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.2 | 3'-phosphoadenylylsulfate + 1-butanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 1-butanoyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + 1-hexanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 1-hexyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + 1-pentanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 1-pentyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + 1-propanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 1-propyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + 11-deoxycorticosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + 2-propanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 2-propyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + 3-methyl-1-butanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 3-methyl-1-butyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + aldosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + aldosterone sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + beta-estradiol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + corticosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + corticosterone sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + cortisol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + cortisol sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + cortisone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + cortisone sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + estriol | poor substrate, relative activity 8% with beta-estradiol as 100% | Rattus norvegicus | adenosine 3',5'-bisphosphate + estriol sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + ethanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + ethyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + methanol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + methyl sulfate | - |
r | |
2.8.2.2 | 3'-phosphoadenylylsulfate + testosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + testosterone 3-sulfate | - |
r | |
2.8.2.2 | additional information | specificity of the enzyme is not limited to hydroxysteroids, a wide range of alcohols serve as sulfate acceptors | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.2.2 | hexamer | 6 * 28000, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.2.2 | hydroxysteroid sulfotransferase 1 | - |
Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.2 | 0.0833 | - |
methanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.133 | - |
aldosterone | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.15 | - |
cortisone | pH 7.5, 37°C cosubstrate ethanol | Rattus norvegicus | |
2.8.2.2 | 0.167 | - |
estriol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.183 | - |
1-propanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.183 | - |
cortisol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.183 | - |
11-deoxycorticosterone | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.217 | - |
2-propanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.283 | - |
1-butanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.417 | - |
1-Hexanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.417 | - |
3-methyl-1-butanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.45 | - |
1-Pentanol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.467 | - |
testosterone | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 0.583 | - |
beta-estradiol | pH 7.5, 37°C | Rattus norvegicus | |
2.8.2.2 | 2 | - |
3beta-hydroxyandrost-5-ene-17-one | pH 7.5, 37°C | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.2 | 5.5 | 6 | beta-estradiol and dehydroepiandrosterone as substrate | Rattus norvegicus |
2.8.2.2 | 7.5 | - |
1-butanol as substrate | Rattus norvegicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.8.2.2 | Rattus norvegicus | preparative isoelectric focusing in sucrose gradients with ampholites in the range pH 3.0-8.0 | - |
5 |