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Literature summary extracted from

  • Lacourciere, G.M.; Stadtman, T.C.
    The NIFS protein can function as a selenide delivery protein in the biosynthesis of selenophosphate (1998), J. Biol. Chem., 273, 30921-30926.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.7 L-selenocysteine inhibits desulfuration of L-cysteine Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.7 0.13
-
 L-selenocysteine 37°C Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine Azotobacter vinelandii
-
 L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
2.8.1.7 additional information Azotobacter vinelandii enzyme serves as a selenide delivery protein for the in vitro biosynthesis of selenophosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.7 Azotobacter vinelandii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.7 0.055
-
 with L-selenocysteine as substrate Azotobacter vinelandii
2.8.1.7 0.142
-
 with L-cysteine as substrate Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine
-
 Azotobacter vinelandii L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
2.8.1.7 L-selenocysteine
-
 Azotobacter vinelandii L-alanine + selenium
-
 ?
2.8.1.7 additional information enzyme serves as a selenide delivery protein for the in vitro biosynthesis of selenophosphate Azotobacter vinelandii ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.7 37
-
 assay at Azotobacter vinelandii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.7 0.04
-
 L-selenocysteine 37°C Azotobacter vinelandii
2.8.1.7 0.1
-
 L-cysteine 37°C Azotobacter vinelandii