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Literature summary extracted from

  • Uhteg, L.; Westley, J.
    Purification and steady-state kinetic analysis of yeast thiosulfate reductase (1979), Arch. Biochem. Biophys., 195, 211-222.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.1.3 freezing and thawing during the later stages of purification destabilize Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.3 benzene thiosulfonate competitive with both substrates Saccharomyces cerevisiae
2.8.1.3 oxidized glutathione product inhibition, competitive with glutathione Saccharomyces cerevisiae
2.8.1.3 sulfate
-
 Saccharomyces cerevisiae
2.8.1.3 Sulfide product inhibition, competitive versus both substrates Saccharomyces cerevisiae
2.8.1.3 sulfite product inhibition, competitive versus both substrates Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.3 17000
-
 SDS-PAGE, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.3 thiosulfate + glutathione Saccharomyces cerevisiae
-
 sulfite + glutathione disulfide + sulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.3 Saccharomyces cerevisiae
-
 Red Star brand
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.3 70fold Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.3 thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide mechanism, substrates add in a random fashion Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.3 62
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.8.1.3 -35°C, concentrated enzyme in solution of 50 mM Tris, pH 8.0, 0.5 mM NaS2O3, 50% glycerol, stable for at least 4 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.3 L-cysteine + glutathione
-
 Saccharomyces cerevisiae ?
-
 ?
2.8.1.3 additional information the enzyme has two distinct closely situated substrate binding sites, one for compounds with an RSO3-structure and one for the sulfhydryl substrate Saccharomyces cerevisiae ?
-
 ?
2.8.1.3 thiosulfate + glutathione
-
 Saccharomyces cerevisiae sulfite + glutathione disulfide + sulfide
-
 ?
2.8.1.3 thiosulfate + glutathione
-
 Saccharomyces cerevisiae sulfite + glutathione disulfide + sulfide the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide ?

Subunits

EC Number Subunits Comment Organism
2.8.1.3 monomer 1 * 17000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.3 9
-
-
 Saccharomyces cerevisiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.1.3 Saccharomyces cerevisiae isoelectric focusing using ampholytes pH 4-6 and pH 2-10
-
 5.1