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Literature summary extracted from

  • Aird, B.A.; Heinrikson, R.L.; Westley, J.
    Isolation and characterization of a prokaryotic sulfurtransferase (1987), J. Biol. Chem., 262, 17327-17335.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.3 sulfate
-
 Acinetobacter calcoaceticus
2.8.1.3 sulfite competitive with respect to dithiothreitol, non-competitive with respect to thiosulfate Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 0.03
-
 CN- 17°C, pH 8.5 Acinetobacter calcoaceticus
2.8.1.1 0.06
-
 CN-
-
 Bos taurus
2.8.1.1 3.2
-
 thiosulfate 17°C, pH 8.5 Acinetobacter calcoaceticus
2.8.1.1 4
-
 thiosulfate
-
 Bos taurus
2.8.1.3 3
-
 cyanide pH 8.5, 27°C Acinetobacter calcoaceticus
2.8.1.3 3.2
-
 thiosulfate pH 8.5, 27°C Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.1 17000
-
 1 * 17000, SDS-PAGE Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Bos taurus
-
 sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide Acinetobacter calcoaceticus
-
 sulfite + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Acinetobacter calcoaceticus
-
 lwoffi
-
2.8.1.1 Bos taurus
-
-
-
2.8.1.3 Acinetobacter calcoaceticus
-
 lwolffi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.1 lwoffi Acinetobacter calcoaceticus
2.8.1.3
-
 Acinetobacter calcoaceticus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.1 thiosulfate + cyanide = sulfite + thiocyanate + H+ double displacement mechanism Bos taurus
a
2.8.1.1 thiosulfate + cyanide = sulfite + thiocyanate + H+ double displacement mechanism Acinetobacter calcoaceticus
a
2.8.1.3 thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide mechanism varies with the acceptor substrate employed Acinetobacter calcoaceticus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.3 1670
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + 2-mercaptoethanol 20% of the activity with cyanide Acinetobacter calcoaceticus ?
-
 ?
2.8.1.1 thiosulfate + a thiol single displacement mechanism Acinetobacter calcoaceticus ?
-
 ?
2.8.1.1 thiosulfate + cyanide
-
 Bos taurus sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide
-
 Acinetobacter calcoaceticus sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + D,L-homocysteine 19% of the activity with cyanide Acinetobacter calcoaceticus ?
-
 ?
2.8.1.1 thiosulfate + dithiothreitol
-
 Acinetobacter calcoaceticus ?
-
 ?
2.8.1.1 thiosulfate + glutathione 20% of the activity with cyanide Acinetobacter calcoaceticus ?
-
 ?
2.8.1.1 thiosulfate + L-cysteine 12% of the activity with cyanide Acinetobacter calcoaceticus ?
-
 ?
2.8.1.1 thiosulfate + N-acetyl-L-cysteine 5% of the activity with cyanide Acinetobacter calcoaceticus ?
-
 ?
2.8.1.3 thiosulfate + cyanide
-
 Acinetobacter calcoaceticus ?
-
 ?
2.8.1.3 thiosulfate + dithiothreitol
-
 Acinetobacter calcoaceticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.1 monomer
-
 Bos taurus
2.8.1.1 monomer 1 * 17000, SDS-PAGE Acinetobacter calcoaceticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.1.3 118
-
 sulfate pH 8.5, 27°C Acinetobacter calcoaceticus