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Literature summary extracted from

  • Turkowsky, A.; Blotevogel, K.H.; Fischer, U.
    Properties of a soluble thiosulfate sulfur transferase (rhodanese) of the marine methanogen Methanosarcina frisia (1991), FEMS Microbiol. Lett., 81, 251-256.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.1.1 dihydrolipoate increases activity at low concentrations Methanosarcina mazei
2.8.1.1 dithioerythritol maximal activation at 0.005 mM, inhibition above Methanosarcina mazei
2.8.1.1 glutathione increases activity at low concentrations Methanosarcina mazei
2.8.1.1 L-cysteine 0.01 mM enhances activity maximally, 0.2 mM: 29% inhibition Methanosarcina mazei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.1 dithioerythritol maximal activation at 0.005 mM, inhibition above Methanosarcina mazei
2.8.1.1 DL-dihydrolipoate activation of the enzyme at 0.001 mM, inhibition above 0.0025 mM Methanosarcina mazei
2.8.1.1 glutathione activation of the enzyme with a maximum at 0.005-0.02 mM, inhibition above Methanosarcina mazei
2.8.1.1 L-cysteine 0.01 mM enhances activity maximally, 0.2 mM: 29% inhibition Methanosarcina mazei
2.8.1.1 lipoate
-
 Methanosarcina mazei
2.8.1.1 N-bromosuccinimide
-
 Methanosarcina mazei
2.8.1.1 SO32-
-
 Methanosarcina mazei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 0.53
-
 thiosulfate 40°C, pH 9.5 Methanosarcina mazei
2.8.1.1 1.57
-
 CN- 40°C, pH 9.5 Methanosarcina mazei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.1 soluble
-
 Methanosarcina mazei
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Methanosarcina mazei
-
 sulfite + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Methanosarcina mazei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.1
-
 Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + cyanide
-
 Methanosarcina mazei sulfite + thiocyanate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.1 60
-
-
 Methanosarcina mazei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.1.1 50
-
 20 min, stable up to, without stabilizer Methanosarcina mazei
2.8.1.1 60
-
 20 min, stable up to, thiosulfate as stabilizer Methanosarcina mazei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.1 11
-
-
 Methanosarcina mazei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.1.1 9 13 stable Methanosarcina mazei