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Literature summary extracted from
- Thiosulfate: cyanide sulfurtransferase (rhodanese) (1981), Methods Enzymol., 77, 285-291.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 2.8.1.1 | - |
Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.1.1 | 2-Naphthalene sulfonate | - |
Bos taurus |  |
| 2.8.1.1 | anions | - |
Bos taurus | |
| 2.8.1.1 | Dinitrobenzene | - |
Bos taurus | |
| 2.8.1.1 | Phenylglyoxal | - |
Bos taurus | |
| 2.8.1.1 | sulfhydryl reagents | - |
Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.8.1.1 | 4 | - |
thiosulfate | 25°C, pH 8.5 | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.8.1.1 | mitochondrion | mammalian liver enzyme occurs exclusively in mitochondrial matrix | Bos taurus | 5739 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.1.1 | thiosulfate + cyanide | Bos taurus | - |
sulfite + thiocyanate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.8.1.1 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.8.1.1 | - |
Bos taurus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 2.8.1.1 | thiosulfate + cyanide = sulfite + thiocyanate + H+ | double displacement mechanism | Bos taurus | |
| 2.8.1.1 | thiosulfate + cyanide = sulfite + thiocyanate + H+ | involving a covalent sulfur-enzyme intermediate | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.8.1.1 | liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 720 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.1.1 | persulfide + cyanide | - |
Bos taurus | ? | - |
? | |
| 2.8.1.1 | thiosulfate + borohydride | - |
Bos taurus | ? | - |
? | |
| 2.8.1.1 | thiosulfate + cyanide | - |
Bos taurus | sulfite + thiocyanate | - |
? | |
| 2.8.1.1 | thiosulfate + dihydrolipoate | - |
Bos taurus | ? | - |
? | |
| 2.8.1.1 | thiosulfate + monothiol | - |
Bos taurus | ? | - |
? | |
| 2.8.1.1 | thiosulfate + sulfite | - |
Bos taurus | sulfite + thiosulfate | - |
? | |
| 2.8.1.1 | thiosulfate + thiosulfinate | - |
Bos taurus | ? | - |
? | |
| 2.8.1.1 | thiosulfonate + cyanide | - |
Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.8.1.1 | More | sedimentation equilibrium studies show that crystalline rhodanese preparation of full specific activity may contain both a nondissociable species of 33000 Da and a 37000 Da species undergoing slow dissociation to species of near 19000 Da | Bos taurus |
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