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Literature summary extracted from

  • Kim, I.Y.; Guimaraes, M.J.; Zlotnik, A.; Bazan, J.F.; Stadtman, T.C.
    Fetal mouse selenophosphate synthetase 2 (SPS2): Characterization of the cysteine mutant form overproduced in a baculovirus-insect cell system (1997), Proc. Natl. Acad. Sci. USA, 94, 418-421.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, expression as N-FLAG tagged protein Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.3 Na+ 5-20 mM Na+ has little effect on enzyme activity in the presence or absence of 1.5 mM K+ Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.3 Mg2+ required Mus musculus
2.7.9.3 Mg2+ the optimal range for the molar ratio of Mg2+ to ATP is between 2:1 and 4:1 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3 recombinant from Sf9 insect cells as N-FLAG tagged protein Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide + H2O specific for ATP Mus musculus AMP + selenophosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.3 37
-
 assay at Mus musculus