Literature summary extracted from
Low, S.C.; Harney, J.W.; Berry, M.J.
Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis (1995), J. Biol. Chem., 270, 21659-21664.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.9.3 |
gene selD, DNA sequence determination and analysis, subcloning and transient expression in enzyme deficient Escherichia coli resulting in poor complemetation of the bacteria by the human gene, and expression in mammalian HtTA cells together with human type I iodothyronine 5'-deiodinase and Xenopus tRNASeC |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.9.3 |
G269C |
site-directed mutagenesis, reduced activity, slightly reduced ATP binding |
Homo sapiens |
2.7.9.3 |
G271R |
site-directed mutagenesis, reduced activity, slightly reduced ATP binding |
Homo sapiens |
2.7.9.3 |
G274A |
site-directed mutagenesis, reduced activity and ATP binding |
Homo sapiens |
2.7.9.3 |
G274D |
site-directed mutagenesis, reduced activity, no ATP binding |
Homo sapiens |
2.7.9.3 |
G274V |
site-directed mutagenesis, no activity and ATP binding |
Homo sapiens |
2.7.9.3 |
H275N |
site-directed mutagenesis, reduced activity, no ATP binding |
Homo sapiens |
2.7.9.3 |
H275Y |
site-directed mutagenesis, increased catalytic activity and ATP binding |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.9.3 |
8-azido-ATP |
competitive to ATP |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.9.3 |
Homo sapiens |
P49903 |
gene selD |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.9.3 |
ATP + selenide + H2O |
- |
Homo sapiens |
AMP + selenophosphate + phosphate |
- |
? |
|