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Literature summary extracted from

  • Veres, Z.; Kim, I.Y.; Scholz, T.D.; Stadtman, T.C.
    Selenophosphate synthetase. Enzyme properties and catalytic reaction (1994), J. Biol. Chem., 269, 10597-10603.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.9.3 3 mM H2O2, pH 7.2, in absence of DTT and EDTA, 50% loss of activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.3 8-azido-ATP competitive to ATP Escherichia coli
2.7.9.3 alpha,beta-methylene-ATP
-
 Escherichia coli
2.7.9.3 AMP competitive to ATP Escherichia coli
2.7.9.3 gamma-Thio-ATP
-
 Escherichia coli
2.7.9.3 Li+ in presence of K+ Escherichia coli
2.7.9.3 Na+ in presence of K+ Escherichia coli
2.7.9.3 phosphate weak Escherichia coli
2.7.9.3 Selenophosphate weak Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.3 0.9
-
 ATP pH 7.2, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.3 K+ monovalent cation required. K+, NH4+ or Rb+ Escherichia coli
2.7.9.3 Mg2+ required Escherichia coli
2.7.9.3 NH4+ monovalent cation required. K+, NH4+ or Rb+ Escherichia coli
2.7.9.3 Rb+ monovalent cation required. K+, NH4+ or Rb+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.3 ATP + selenide + H2O Escherichia coli the enzyme provides the biologically active selenium donor compound monoseleniumphosphate AMP + selenophosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.9.3 -80°C, stable for 1 year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide + H2O specific for ATP Escherichia coli AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide + H2O in the absence of selenide, ATP is converted completely to AMP and phosphate Escherichia coli AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide + H2O the enzyme provides the biologically active selenium donor compound monoseleniumphosphate Escherichia coli AMP + selenophosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.3 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.9.3 22
-
 pH 5, 10 min, stable Escherichia coli
2.7.9.3 60
-
 pH 7.2, aerobic conditions, 5 min, stable Escherichia coli
2.7.9.3 70
-
 pH 7.2, aerobic conditions, 5 min, complete inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.3 7.2 9
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.9.3 5
-
 22°C, 10 min, stable Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.9.3 0.17
-
 AMP pH 7.2, 37°C Escherichia coli