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Literature summary extracted from

  • Chastain, C.J.; Botschner, M.; Harrington, G.E.; Thompson, B.J.; Mills, S.E.; Sarath, G.; Chollet, R.
    Further analysis of maize C(4) pyruvate,orthophosphate dikinase phosphorylation by its bifunctional regulatory protein using selective substitutions of the regulatory Thr-456 and catalytic His-458 residues (2000), Arch. Biochem. Biophys., 375, 165-170.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1 expressed in Escherichia coli Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.1 H458N no activity Zea mays
2.7.9.1 T456E no activity Zea mays
2.7.9.1 T456F
-
 Zea mays
2.7.9.1 T456Y
-
 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 95000
-
 x * 95000 immunoblot analysis Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.27 Zea mays
-
-
-
2.7.9.1 Zea mays
-
-
-
2.7.11.32 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.27 leaf
-
 Zea mays
-
2.7.11.32 leaf
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 0.043
-
 T456F mutant Zea mays
2.7.9.1 0.24
-
 T456Y mutant Zea mays
2.7.9.1 4.1
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.27 [pyruvate,phosphate dikinase]phosphate + phosphate His458 and Thr456 phosphorylated (inactive) Zea mays pyruvate,phosphate dikinase + diphosphate His458 phosphorylated (active) ?
2.7.9.1 ATP + pyruvate + phosphate
-
 Zea mays AMP + phosphoenolpyruvate + diphosphate
-
 r
2.7.11.32 pyruvate,phosphate dikinase + ADP His458 phosphorylated (activ), substrate mutant proteins T456Y and H458N are not phosphorylated (mutant protein T456S is phosphorylated), therefore a phosphorylated His458 in the substrate protein is necessary and the regulatory protein is functionally a Ser/Thr protein kinase Zea mays [pyruvate,phosphate dikinase]phosphate + AMP His458 and Thr456 phosphorylated (inactive) ?

Subunits

EC Number Subunits Comment Organism
2.7.9.1 ? x * 95000 immunoblot analysis Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.11.32 bifunctional regulatory protein
-
 Zea mays