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Literature summary extracted from

  • Hiltpold, A.; Thomas, R.M.; Kohler, P.
    Purification and characterization of recombinant pyruvate phosphate dikinase from Giardia (1999), Mol. Biochem. Parasitol., 104, 157-169.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1 expressed in a baculovirus system Giardia intestinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 biphosphonate mixed inhibition mechanism with respect to diphosphate Giardia intestinalis
2.7.9.1 diphosphate
-
 Giardia intestinalis
2.7.9.1 Imidodiphosphate competitive to diphosphate Giardia intestinalis
2.7.9.1 oxalate mixed mechanism with respect to phosphoenolpyruvate and diphosphate Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 0.0013
-
 AMP pH 7, 30ºC Giardia intestinalis
2.7.9.1 0.029
-
 diphosphate pH 7, 30ºC Giardia intestinalis
2.7.9.1 0.032
-
 ATP pH 7, 30ºC Giardia intestinalis
2.7.9.1 0.033
-
 phosphoenolpyruvate pH 7, 30ºC Giardia intestinalis
2.7.9.1 0.075
-
 pyruvate pH 7, 30ºC Giardia intestinalis
2.7.9.1 1.8
-
 phosphate pH 7, 30ºC Giardia intestinalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 Mg2+ activity increases with increasing Mg2+ concentrations, reaching a plateau at 2-4 mM. At higher concentrations the effect begins to reverse Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 97600
-
 2 * 97600, analytical ultracentrifugation and gel filtration Giardia intestinalis
2.7.9.1 195000
-
 gel filtration Giardia intestinalis
2.7.9.1 198400
-
 gel electrophoresis Giardia intestinalis

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Giardia intestinalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
 Giardia intestinalis AMP + phosphoenolpyruvate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.1 dimer 2 * 97600, analytical ultracentrifugation and gel filtration Giardia intestinalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.9.1 0.001
-
 oxalate pH 7, 30ºC, less than 0.001 mM Giardia intestinalis
2.7.9.1 0.55
-
 diphosphate pH 7, 30ºC Giardia intestinalis