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Literature summary extracted from

  • Smith, C.M.; Duff, S.M.; Chollet, R.
    Partial purification and characterization of maize-leaf pyruvate, orthophosphate dikinase regulatory protein: a low-abundance, mesophyll-chloroplast stromal protein (1994), Arch. Biochem. Biophys., 308, 200-206.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.27 chloroplast mesophyll-chloroplast stroma Zea mays 9507
-
2.7.9.1 chloroplast
-
Zea mays 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.27 48000
-
2 * 48000, SDS-PAGE, gel filtration, pH 7.0-7.5 Zea mays
2.7.4.27 48000
-
4 * 48000, SDS-PAGE, gel filtration, pH 8.3 Zea mays
2.7.4.27 90000
-
gel filtration, pH 7.0-7.5 Zea mays
2.7.4.27 180000
-
gel filtration, pH 8.3 Zea mays
2.7.9.1 94000
-
x * 94000, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.27 Zea mays
-
-
-
2.7.9.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.27 partially purified, ammonium sulfate precipitation, dye-ligand chromatography (Blue Sepharose), gel filtration, anion exchange chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.27 leaf
-
Zea mays
-
2.7.9.1 mesophyll
-
Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.27 [pyruvate,phosphate dikinase]phosphate + phosphate Thr456 phosphorylated (inactive) Zea mays pyruvate,phosphate dikinase + diphosphate active ?

Subunits

EC Number Subunits Comment Organism
2.7.4.27 homodimer 2 * 48000, SDS-PAGE, gel filtration, pH 7.0-7.5 Zea mays
2.7.4.27 homotetramer 4 * 48000, SDS-PAGE, gel filtration, pH 8.3 Zea mays
2.7.9.1 ? x * 94000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.4.27 pyruvate,orthophosphate dikinase regulatory protein
-
Zea mays