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Literature summary extracted from
- Pyruvate, orthophosphate dikinase from Bacteroides symbiosus (1975), Methods Enzymol., 42C, 187-191.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.9.1 | Ca2+ | - |
[Clostridium] symbiosum |  |
| 2.7.9.1 | CTP | total inhibition of the forward reaction at 1 mM | [Clostridium] symbiosum | |
| 2.7.9.1 | ITP | total inhibition of the forward reaction at 1 mM | [Clostridium] symbiosum | |
| 2.7.9.1 | additional information | no tested 5'-nucleoside monophosphate inhibits the reaction with AMP | [Clostridium] symbiosum | |
| 2.7.9.1 | oxalate | - |
[Clostridium] symbiosum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.9.1 | 0.0035 | - |
AMP | - |
[Clostridium] symbiosum | |
| 2.7.9.1 | 0.08 | - |
pyruvate | pH 6.8, 25ºC, pyruvate formation | [Clostridium] symbiosum | |
| 2.7.9.1 | 0.1 | - |
ATP | pH 6.8, 25ºC, pyruvate formation | [Clostridium] symbiosum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.1 | 94000 | - |
x * 94000, SDS-PAGE | [Clostridium] symbiosum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.9.1 | additional information | [Clostridium] symbiosum | functions in glycolytic pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.9.1 | [Clostridium] symbiosum | - |
ATCC 14940 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.9.1 | partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel | [Clostridium] symbiosum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.1 | additional information | - |
- |
[Clostridium] symbiosum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.9.1 | ATP + pyruvate + phosphate | specificity is strictly restricted to adenine nucleotides | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
| 2.7.9.1 | additional information | functions in glycolytic pathway | [Clostridium] symbiosum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.9.1 | ? | x * 94000, SDS-PAGE | [Clostridium] symbiosum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.1 | 30 | - |
assay at | [Clostridium] symbiosum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.1 | 6.4 | - |
reverse reaction, pyruvate formation | [Clostridium] symbiosum |
| 2.7.9.1 | 7.2 | 8 | forward reaction, phosphoenolpyruvate formation | [Clostridium] symbiosum |
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