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Literature summary extracted from

  • Mofid, M.R.; Finking, R.; Marahiel, M.A.
    Recognition of hybrid peptidyl carrier proteins/acyl carrier proteins in nonribosomal peptide synthetase modules by the 4'-phosphopantetheinyl transferases AcpS and Sfp (2002), J. Biol. Chem., 277, 17023-17031.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 sfp and acpS cloned and overproduced in Escherichia coli M15 Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.8.7 co-crystallized with CoA and ACP Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.0002
-
 apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme AcpS, 0.002-0.008 mM substrate Bacillus subtilis
2.7.8.7 0.0014
-
 apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme Sfp, 0.002-0.008 mM substrate Bacillus subtilis
2.7.8.7 0.00445
-
 apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp Bacillus subtilis
2.7.8.7 0.0216
-
 apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme AcpS, apo-hPCP Bacillus subtilis
2.7.8.7 0.026
-
 apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp, apo-hPCP Bacillus subtilis
2.7.8.7 0.038
-
 apo-ACP pH 8.8, 37°C, isoenzyme Sfp, 0.02-0.2 mM substrate Bacillus subtilis
2.7.8.7 0.068
-
 apo-ACP pH 8.8, 37°C, isoenzyme AcpS, 0.02-0.2 mM substrate Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Bacillus subtilis
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant isoenzymes AcpS and Sfp, expressed in Escherichia coli Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[peptidyl carrier protein]
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
 r
2.7.8.7 additional information Sfp type exhibits an extraordinarily broad substrate specificity Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.7 monomer isoenzyme AcpS Bacillus subtilis
2.7.8.7 trimer isoenzyme Sfp Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 0.0283
-
 apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme Sfp, 0.002-0.008 mM substrate Bacillus subtilis
2.7.8.7 0.208
-
 apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme Sfp, 0.02-0.2 mM substrate Bacillus subtilis
2.7.8.7 0.239
-
 apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme AcpS, apo-hPCP Bacillus subtilis
2.7.8.7 0.367
-
 apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme AcpS, 0.002-0.008 mM substrate Bacillus subtilis
2.7.8.7 1.6
-
 apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp Bacillus subtilis
2.7.8.7 1.6
-
 apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp, apo-hPCP Bacillus subtilis
2.7.8.7 2.08
-
 apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme AcpS, 0.02-0.2 mM substrate Bacillus subtilis