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Literature summary extracted from

  • Quadri, L.E.; Weinreb, P.H.; Lei, M.; Nakano, M.M.; Zuber, P.; Walsh, C.T.
    Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases (1998), Biochemistry, 37, 1585-1595.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 cloned in Escherichia coli DH5alpha and overexpressed in Escherichia coli MV1190/pUC8-Sfp Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.7 D107A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
2.7.8.7 D107E site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
2.7.8.7 E151A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
2.7.8.7 G105A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
2.7.8.7 G105D site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
2.7.8.7 K155A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
2.7.8.7 W147A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
2.7.8.7 W147F site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.7 additional information no substantial inhibition of Sfp by apo-[PCPH6SrfB1.18] Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.0018
-
apo-[PCPH6SrfB2.18] pH 6.0, 37°C Bacillus subtilis
2.7.8.7 0.005
-
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae] pH 6.0, 37°C Bacillus subtilis
2.7.8.7 0.006
-
apo-acyl-carrier protein pH 6.0, 37°C Bacillus subtilis
2.7.8.7 0.007
-
CoA pH 6.0, 37°C Bacillus subtilis
2.7.8.7 0.016
-
apo-[EntB-ArCP-H6 Escherichia coli] pH 6.0, 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Bacillus subtilis enzyme Sfp required for production of the lipoheptapeptide antibiotic surfactin adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant enzyme, expressed in Escherichia coli MV1190/pUC8-Sfp Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 acetonyldethio-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
2.7.8.7 acetyl-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis CoA + acetyl-[acyl-carrier protein]
-
r
2.7.8.7 benzoyl-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis CoA + benzoyl-[acyl-carrier protein]
-
r
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
2.7.8.7 CoA + apo-[acyl-carrier protein] enzyme Sfp required for production of the lipoheptapeptide antibiotic surfactin Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
2.7.8.7 CoA + apo-[EntB-ArCP-H6 E. coli]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
2.7.8.7 CoA + apo-[Lys2-PCP-H6 Saccharomyces cerevisiae]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
2.7.8.7 CoA + apo-[PCPH6SrfB1.18]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
2.7.8.7 CoA + apo-[PCPH6SrfB2.18]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[EntB-ArCP-H6 Escherichia coli]
-
Bacillus subtilis ?
-
?
2.7.8.7 desulfo-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
2.7.8.7 homocysteamine-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
2.7.8.7 additional information 3'-dephospho-CoA is no substrate Bacillus subtilis ?
-
?
2.7.8.7 phenylacetyl-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 phosphopantetheinyl transferase
-
Bacillus subtilis
2.7.8.7 PPTase
-
Bacillus subtilis
2.7.8.7 Sfp
-
Bacillus subtilis
2.7.8.7 surfactin synthetase
-
Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 0.0967
-
apo-acyl-carrier protein pH 6.0, 33°C Bacillus subtilis
2.7.8.7 0.933
-
apo-[PCPH6SrfB2.18] pH 6.0, 37°C Bacillus subtilis
2.7.8.7 1.08
-
apo-[EntB-ArCP-H6 Escherichia coli] pH 6.0, 37°C Bacillus subtilis
2.7.8.7 1.17
-
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae] pH 6.0, 37°C Bacillus subtilis
2.7.8.7 1.7
-
apo-acyl-carrier protein pH 6.0, 37°C Bacillus subtilis
2.7.8.7 1.73
-
apo-[PCPH6SrfB1.18] pH 6.0, 37°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 6
-
-
Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.7 4.5 8.5 less than 20% of maximum activity at both pH 5.0 and 7.0, 50% of activity maximum at pH 5.5 and pH 6.8 Bacillus subtilis