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Literature summary extracted from

  • Elhussein, S.A.; Miernyk, J.A.; Ohlrogge, J.B.
    Plant holo-(acyl carrier protein) synthase (1988), Biochem. J., 252, 39-45.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.8.7 quite heat labile, unstable during chromatographic procedures intended for its purification, can only be partially overcome by Mg2+ and dithiothreitol, addition of ethylene glycol, glycerol, EDTA or proteinase inhibitors failed to stabilize the plant enzyme Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.7 3',5'-ADP inhibited in vitro by the reaction product Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.002
-
 apo-acyl-carrier protein pH 8.0, 37°C Spinacia oleracea
2.7.8.7 0.072
-
 apo-acyl-carrier protein pH 8.0, 37°C Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.7 cytosol
-
 Spinacia oleracea 5829
-
2.7.8.7 cytosol
-
 Ricinus communis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+ requires Mg2+ or Mn2+ for activity, Km for Mg2+ 3.0 mM Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Spinacia oleracea
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] Ricinus communis
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Ricinus communis
-
 castor-oil-seed, L. cv. Baker 296
-
2.7.8.7 Spinacia oleracea
-
 spinach, L. cv. 424
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 partially Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.7 endosperm
-
 Ricinus communis
-
2.7.8.7 leaf
-
 Spinacia oleracea
-
2.7.8.7 seed
-
 Ricinus communis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Spinacia oleracea adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Ricinus communis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 additional information specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive Spinacia oleracea ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 8.2
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.7 7 8.8 half maximal activity at pH 7.4 Spinacia oleracea