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Literature summary extracted from

  • Zeng, Y.; Elbein, A.D.
    UDP-N-acetylglucosamine:dolichyl-phosphate N-acetylglucosamine-1-phosphate transferase is amplified in tunicamycin-resistant soybean cells (1995), Eur. J. Biochem., 233, 458-466.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.15 ATP 0.01-0.025 mM, stimulates activity of tunicamycin-resistant cells about 20-30% Glycine max
2.7.8.15 phosphatidylglycerol activity is slightly enhanced Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.15 GDP-mannose slight Glycine max
2.7.8.15 GTP 0.4 mM, 25-30% inhibition, enzyme from tunicamycin-resistant cells, no inhibition of wild-type enzyme up to 0.4 mM Glycine max
2.7.8.15 PCMB the enzyme from tunicamycin-resistant cells is equally sensitive to tunicamycin as the wilde-type enzyme Glycine max
2.7.8.15 UDP-GlcNAc strong inhibition Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.15 39000
-
 x * 39000, SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.15 Glycine max
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.15 cell culture the tunicamycin resistant cells have a greater than 40fold increase in the activity of the enzyme UDP-GlcNAc:dolichyl-P GlcNAc1P transferase. Increase in enzyme activity is due to an increased production of the enzyme Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.15 UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
 Glycine max UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.15 ? x * 39000, SDS-PAGE Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.8.15 35
-
 the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme Glycine max
2.7.8.15 40
-
 the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme Glycine max