EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.8.11 | medicine | enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.11 | tbINO, the coding sequence of ino1 amplified by PCR, cloned into expression vector pET-15b and overproduced in Escherichia coli BL21 (DE3) | Mycobacterium tuberculosis |
5.5.1.4 | - |
Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.8.11 | bound to the cofactor NAD+, space group P42(1)2, cell dimensions a = 116.2, b = 116.2, c = 64.5 | Mycobacterium tuberculosis |
5.5.1.4 | hanging-drop vapour-diffusion method, crystal structure of native and selenomethionine-bound enzyme at 1.95 resolution, bound to the cofactor NAD+ | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.4 | Zn2+ | enzyme contains Zn2+. The nicotinamide moiety of the NAD+ cofactor is in the syn conformation about the N-glycosidic bond and is held there by coordination with the Zn2+ ion. N233, D235 and D310 provide ligands to a water molecule that coordinates the zinc ion | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.11 | CDP-diacylglycerol + myo-inositol | Mycobacterium tuberculosis | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.11 | Mycobacterium tuberculosis | - |
- |
- |
5.5.1.4 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.11 | recombinant enzyme | Mycobacterium tuberculosis |
5.5.1.4 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Mycobacterium tuberculosis | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
5.5.1.4 | D-glucose 6-phosphate | key enzyme in inositol biosynthesis, rate-limiting step | Mycobacterium tuberculosis | 1D-inositol 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.5.1.4 | tbINO | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.11 | NAD+ | - |
Mycobacterium tuberculosis | |
5.5.1.4 | NAD+ | - |
Mycobacterium tuberculosis |