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Literature summary extracted from
- Phosphatidylinositol synthase from mammalian tissues (1997), Biochim. Biophys. Acta, 1348, 179-186.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.8.11 | cDNA cloned by functional complementation of a Saccharomyces cerevisiae mutant | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | Ca2+ | - |
Bos taurus |  |
| 2.7.8.11 | Ca2+ | - |
Cavia porcellus | |
| 2.7.8.11 | Ca2+ | - |
Homo sapiens | |
| 2.7.8.11 | Ca2+ | - |
Oryctolagus cuniculus | |
| 2.7.8.11 | Ca2+ | - |
Rattus norvegicus | |
| 2.7.8.11 | CDP-diacylglycerol | above 0.5 mM | Homo sapiens | |
| 2.7.8.11 | CDP-diacylglycerol | above 0.4 mM | Rattus norvegicus | |
| 2.7.8.11 | diphosphate | - |
Homo sapiens | |
| 2.7.8.11 | hexachlorocyclohexane | inhibition is non-competitive and not specific for inositol processing enzymes | Homo sapiens | |
| 2.7.8.11 | additional information | AMP CMP, GMP, UMP, TMP, TDP, and GDP are not inhibitory, triphosphates are even more inhibitory than diphosphates; no inhibition with 2-deoxy-CDP-diacylglycerol | Homo sapiens | |
| 2.7.8.11 | phosphate | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.8.11 | 0.0095 | - |
CDP-diacylglycerol | - |
Rattus norvegicus | |
| 2.7.8.11 | 0.036 | - |
CDP-diacylglycerol | - |
Homo sapiens | |
| 2.7.8.11 | 0.045 | - |
2-deoxy-CDP-diacylglycerol | - |
Homo sapiens | |
| 2.7.8.11 | 0.28 | - |
myo-inositol | - |
Homo sapiens | |
| 2.7.8.11 | 1.3 | - |
myo-inositol | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.8.11 | endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| 2.7.8.11 | endoplasmic reticulum | - |
Oryctolagus cuniculus | 5783 | - |
| 2.7.8.11 | endoplasmic reticulum | localized to both the rough and the smooth endoplasmic reticulum | Rattus norvegicus | 5783 | - |
| 2.7.8.11 | Golgi apparatus | - |
Homo sapiens | 5794 | - |
| 2.7.8.11 | Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
| 2.7.8.11 | membrane | - |
Gallus gallus | 16020 | - |
| 2.7.8.11 | membrane | - |
Cavia porcellus | 16020 | - |
| 2.7.8.11 | membrane | membrane-bound | Homo sapiens | 16020 | - |
| 2.7.8.11 | membrane | membrane-bound | Rattus norvegicus | 16020 | - |
| 2.7.8.11 | membrane | membrane-bound | Saccharomyces cerevisiae | 16020 | - |
| 2.7.8.11 | microsome | - |
Cavia porcellus | - |
- |
| 2.7.8.11 | microsome | - |
Homo sapiens | - |
- |
| 2.7.8.11 | microsome | - |
Rattus norvegicus | - |
- |
| 2.7.8.11 | microsome | - |
Saccharomyces cerevisiae | - |
- |
| 2.7.8.11 | microsome | - |
Bos taurus | - |
- |
| 2.7.8.11 | microsome | - |
Oryctolagus cuniculus | - |
- |
| 2.7.8.11 | plasma membrane | - |
Rattus norvegicus | 5886 | - |
| 2.7.8.11 | plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
| 2.7.8.11 | plasma membrane | proximal tubule cells | Oryctolagus cuniculus | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | Mg2+ | - |
Cavia porcellus | |
| 2.7.8.11 | Mg2+ | - |
Homo sapiens | |
| 2.7.8.11 | Mg2+ | - |
Rattus norvegicus | |
| 2.7.8.11 | Mg2+ | - |
Saccharomyces cerevisiae | |
| 2.7.8.11 | Mg2+ | - |
Bos taurus | |
| 2.7.8.11 | Mg2+ | - |
Oryctolagus cuniculus | |
| 2.7.8.11 | Mn2+ | - |
Cavia porcellus | |
| 2.7.8.11 | Mn2+ | - |
Homo sapiens | |
| 2.7.8.11 | Mn2+ | - |
Rattus norvegicus | |
| 2.7.8.11 | Mn2+ | - |
Saccharomyces cerevisiae | |
| 2.7.8.11 | Mn2+ | - |
Bos taurus | |
| 2.7.8.11 | Mn2+ | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 21000 | - |
? * 21000, SDS-PAGE | Rattus norvegicus |
| 2.7.8.11 | 23610 | - |
- |
Rattus norvegicus |
| 2.7.8.11 | 24000 | - |
SDS-PAGE | Homo sapiens |
| 2.7.8.11 | 34000 | - |
- |
Rattus norvegicus |
| 2.7.8.11 | 34000 | - |
- |
Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Gallus gallus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Meleagris gallopavo | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Cavia porcellus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Homo sapiens | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Saccharomyces cerevisiae | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Oryctolagus cuniculus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Rattus norvegicus | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Bos taurus | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.8.11 | Bos taurus | - |
- |
- |
| 2.7.8.11 | Cavia porcellus | - |
guinea pig | - |
| 2.7.8.11 | Gallus gallus | - |
chicken | - |
| 2.7.8.11 | Homo sapiens | - |
human | - |
| 2.7.8.11 | Meleagris gallopavo | - |
turkey | - |
| 2.7.8.11 | Oryctolagus cuniculus | - |
- |
- |
| 2.7.8.11 | Rattus norvegicus | - |
rat | - |
| 2.7.8.11 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.8.11 | - |
Homo sapiens |
| 2.7.8.11 | - |
Rattus norvegicus |
| 2.7.8.11 | - |
Saccharomyces cerevisiae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.8.11 | brain | - |
Gallus gallus | - |
| 2.7.8.11 | brain | - |
Cavia porcellus | - |
| 2.7.8.11 | brain | - |
Rattus norvegicus | - |
| 2.7.8.11 | brain | - |
Bos taurus | - |
| 2.7.8.11 | cerebral cortex | - |
Oryctolagus cuniculus | - |
| 2.7.8.11 | erythrocyte | - |
Meleagris gallopavo | - |
| 2.7.8.11 | glioma cell | - |
Oryctolagus cuniculus | - |
| 2.7.8.11 | heart | - |
Gallus gallus | - |
| 2.7.8.11 | heart | - |
Cavia porcellus | - |
| 2.7.8.11 | kidney | - |
Cavia porcellus | - |
| 2.7.8.11 | liver | - |
Gallus gallus | - |
| 2.7.8.11 | liver | - |
Cavia porcellus | - |
| 2.7.8.11 | liver | - |
Rattus norvegicus | - |
| 2.7.8.11 | lung | - |
Cavia porcellus | - |
| 2.7.8.11 | lung | - |
Oryctolagus cuniculus | - |
| 2.7.8.11 | neuroblastoma cell | - |
Oryctolagus cuniculus | - |
| 2.7.8.11 | pancreas | - |
Cavia porcellus | - |
| 2.7.8.11 | pituitary gland tumor cell | GH3 tumour | Rattus norvegicus | - |
| 2.7.8.11 | placenta | - |
Homo sapiens | - |
| 2.7.8.11 | spleen | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 2.8 | - |
- |
Rattus norvegicus |
| 2.7.8.11 | 34 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | 2-deoxy-CDP-diacylglycerol + myo-inositol | optimal activity is 25% higher then with CDP-diacylglycerol | Homo sapiens | CMP + ? | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Gallus gallus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Meleagris gallopavo | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Cavia porcellus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Homo sapiens | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Rattus norvegicus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Saccharomyces cerevisiae | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Bos taurus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Oryctolagus cuniculus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | Rattus norvegicus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | Bos taurus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 2.7.8.11 | additional information | UDP-diacylglycerol, ADP-diacylglycerol, and GDP-diacylglycerol are inactive as substrates | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.8.11 | ? | - |
Saccharomyces cerevisiae |
| 2.7.8.11 | ? | ? * 21000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 8.5 | 9 | - |
Bos taurus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.8.11 | 0.2 | - |
diphosphate | - |
Homo sapiens | |
| 2.7.8.11 | 0.8 | - |
phosphate | - |
Homo sapiens | |
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