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Literature summary extracted from
- Purification and characterization of phosphatidylinositol synthase from human placenta (1994), Biochem. J., 297, 517-522.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | Triton X-100 | - |
Homo sapiens |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | ADP | - |
Homo sapiens | |
| 2.7.8.11 | ATP | - |
Homo sapiens | |
| 2.7.8.11 | Ca2+ | - |
Homo sapiens | |
| 2.7.8.11 | CaCl2 | - |
Homo sapiens | |
| 2.7.8.11 | CDP | competitive inhibition | Homo sapiens | |
| 2.7.8.11 | CTP | - |
Homo sapiens | |
| 2.7.8.11 | diphosphate | - |
Homo sapiens | |
| 2.7.8.11 | GTP | - |
Homo sapiens | |
| 2.7.8.11 | additional information | AMP CMP, GMP, UMP, TMP, TDP, and GDP are not inhibitory, triphosphates are even more inhibitory than diphosphates | Homo sapiens | |
| 2.7.8.11 | phosphate | - |
Homo sapiens | |
| 2.7.8.11 | TTP | - |
Homo sapiens | |
| 2.7.8.11 | UDP | - |
Homo sapiens | |
| 2.7.8.11 | UTP | - |
Homo sapiens | |
| 2.7.8.11 | Zn2+ | - |
Homo sapiens | |
| 2.7.8.11 | ZnCl2 | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.8.11 | 0.28 | - |
myo-inositol | pH 9.0, 37°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.8.11 | endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| 2.7.8.11 | Golgi apparatus | - |
Homo sapiens | 5794 | - |
| 2.7.8.11 | membrane | membrane-bound | Homo sapiens | 16020 | - |
| 2.7.8.11 | microsome | - |
Homo sapiens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | Mg2+ | - |
Homo sapiens | |
| 2.7.8.11 | Mn2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 24000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Homo sapiens | key enzyme in cell membrane biosynthesis | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.8.11 | Homo sapiens | - |
human | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.8.11 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.8.11 | placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 34 | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.7.8.11 | -20°C, frozen and stored or frozen in liquid nitrogen and subsequently stored, over 50% activity is lost after 24 h | Homo sapiens |
| 2.7.8.11 | -60°C, frozen and stored or frozen in liquid nitrogen and subsequently stored, over 50% activity is lost after 24 h | Homo sapiens |
| 2.7.8.11 | -80°C, regularly frozen in liqid N2, no detectable loss of activity is experienced during storage at -80°C for up to 2 months | Homo sapiens |
| 2.7.8.11 | 4°C, stored on ice, 10% of the activity is lost during 24 h | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | 2-deoxy-CDP-diacylglycerol + myo-inositol | - |
Homo sapiens | CMP + ? | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Homo sapiens | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | key enzyme in cell membrane biosynthesis | Homo sapiens | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 50 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 50 | - |
half-life without substrates 20 min | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 8 | - |
in Tris/HCl buffer, maximal activity is 60% of the activity in glycylglycine/NaOH at pH 9.0, at pH 9.0 almost no activity in Tris/HCl buffer | Homo sapiens |
| 2.7.8.11 | 9 | - |
in glycylglycine/NaOH or glycine/NaOH buffer | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.8.11 | 0.2 | - |
diphosphate | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 0.6 | - |
phosphate | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 0.6 | - |
CaCl2 | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 0.8 | - |
phosphate | - |
Homo sapiens | |
| 2.7.8.11 | 1.4 | - |
ATP | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 1.4 | - |
UTP | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 1.4 | - |
TTP | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 1.6 | - |
GTP | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 1.8 | - |
CTP | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 2 | - |
ZnCl2 | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 3.8 | - |
UDP | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 4 | - |
CDP | pH 9.0, 37°C | Homo sapiens | |
| 2.7.8.11 | 4.1 | - |
ADP | pH 9.0, 37°C | Homo sapiens | |
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