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Literature summary extracted from

  • Bravo, I.G.; Barrallo, S.; Ferrero, M.A.; Rodriguez-Aparicio, L.B.; Martinez-Blanco, H.; Reglero, A.
    Kinetic properties of the acylneuraminate cytidylyltransferase from Pasteurella haemolytica A2 (2001), Biochem. J., 358, 585-598.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.43 ATP 1 mM, 20% activation Mannheimia haemolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Mannheimia haemolytica
2.7.7.43 AMP 10 mM, 64% inhibition. 20% inhibition by 1 mM Mannheimia haemolytica
2.7.7.43 ATP 10 mM, 60% inhibition Mannheimia haemolytica
2.7.7.43 Ba2+ 20 mM, less than 30% inhibition Mannheimia haemolytica
2.7.7.43 Ca2+ 20 mM in presence of 10 mM Mg2+, more than 90% inhibition Mannheimia haemolytica
2.7.7.43 CDP
-
 Mannheimia haemolytica
2.7.7.43 CMP 50 mM, 58% inhibition Mannheimia haemolytica
2.7.7.43 Co2+ 20 mM in presence of 10 mM Mg2+, more than 70% inhibition Mannheimia haemolytica
2.7.7.43 CTP
-
 Mannheimia haemolytica
2.7.7.43 Cu2+ 20 mM in presence of 10 mM Mg2+, more than 90% inhibition Mannheimia haemolytica
2.7.7.43 cytidine 60 mM, 37% inhibition Mannheimia haemolytica
2.7.7.43 Fe3+ 20 mM in presence of 10 mM Mg2+, more than 90% inhibition Mannheimia haemolytica
2.7.7.43 GMP 20 mM, 64% inhibition Mannheimia haemolytica
2.7.7.43 GTP 10 mM, 39% inhibition Mannheimia haemolytica
2.7.7.43 Hg2+ 20 mM in presence of 10 mM Mg2+, more than 90% inhibition Mannheimia haemolytica
2.7.7.43 Mn2+ 20 mM in presence of 10 mM Mg2+, more than 90% inhibition Mannheimia haemolytica
2.7.7.43 NEM
-
 Mannheimia haemolytica
2.7.7.43 Ni2+ 20 mM in presence of 10 mM Mg2+, more than 90% inhibition Mannheimia haemolytica
2.7.7.43 PHMB 1 mM, complete inhibition Mannheimia haemolytica
2.7.7.43 Sr2+ 20 mM, less than 30% inhibition Mannheimia haemolytica
2.7.7.43 UDP-N-acetylglucosamine 10 mM, 26% inhibition Mannheimia haemolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.36
-
 CTP pH 9.0, 37°C, reaction with N-glycolylneuraminate Mannheimia haemolytica
2.7.7.43 1.3
-
 N-acetylneuraminate methyl ester pH 9.0, 37°C, reaction with CTP Mannheimia haemolytica
2.7.7.43 1.6
-
 N-acetylneuraminate pH 9.0, 37°C, reaction with TTP Mannheimia haemolytica
2.7.7.43 1.77
-
 CTP pH 9.0, 37°C, reaction with N-acetylneuraminate Mannheimia haemolytica
2.7.7.43 1.8
-
 N-acetylneuraminate pH 9.0, 37°C, reaction with CDP Mannheimia haemolytica
2.7.7.43 1.82
-
 N-acetylneuraminate pH 9.0, 37°C, reaction with CTP Mannheimia haemolytica
2.7.7.43 1.9
-
 N-acetylneuraminate pH 9.0, 37°C, reaction with UTP Mannheimia haemolytica
2.7.7.43 1.9
-
 N-acetylneuraminate pH 9.0, 37°C, reaction with UDP Mannheimia haemolytica
2.7.7.43 2.1
-
 CDP pH 9.0, 37°C, reaction with N-acetylneuraminate Mannheimia haemolytica
2.7.7.43 2.6
-
 CTP pH 9.0, 37°C, reaction with N-acetylneuraminate methyl ester Mannheimia haemolytica
2.7.7.43 2.7
-
 UTP pH 9.0, 37°C, reaction with N-acetylneuraminate Mannheimia haemolytica
2.7.7.43 3
-
 N-glycolylneuraminate pH 9.0, 37°C, reaction with CTP Mannheimia haemolytica
2.7.7.43 3
-
 UDP pH 9.0, 37°C, reaction with N-acetylneuraminate Mannheimia haemolytica
2.7.7.43 8.1
-
 TTP pH 9.0, 37°C, reaction with N-acetylneuraminate Mannheimia haemolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Ca2+ divalent metal ion is strictly required, with 20 mM Ca2+ 13% of the activation compared to activation by Mg2+ Mannheimia haemolytica
2.7.7.43 Co2+ divalent metal ion is strictly required, with 20 mM Mn2+ 27% of the activation compared to activation by Mg2+ Mannheimia haemolytica
2.7.7.43 Mg2+ divalent metal ion is strictly required, maximal activity at 10 mM Mannheimia haemolytica
2.7.7.43 Mn2+ divalent metal ion is strictly required, with 20 mM Mn2+ 28% of the activation compared to activation by Mg2+ Mannheimia haemolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.43 43000
-
 3 * 43000, SDS-PAGE Mannheimia haemolytica
2.7.7.43 135000
-
 gel filtration Mannheimia haemolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate Mannheimia haemolytica neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate Mannheimia haemolytica A2 neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase diphosphate + CMP-N-acylneuraminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Mannheimia haemolytica
-
 A2
-
2.7.7.43 Mannheimia haemolytica A2
-
 A2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Mannheimia haemolytica

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.43 CTP + N-acylneuraminate = diphosphate + CMP-N-acylneuraminate ping pong bi bi mechanism with CTP as the first substrate and CMP-NeuAc as the last product Mannheimia haemolytica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CDP + N-acetylneuraminate
-
 Mannheimia haemolytica phosphate + CMP-N-acetylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Mannheimia haemolytica diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase Mannheimia haemolytica diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Mannheimia haemolytica A2 diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase Mannheimia haemolytica A2 diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate methyl ester
-
 Mannheimia haemolytica diphosphate + CMP-N-acylneuraminate methyl ester
-
 ?
2.7.7.43 CTP + N-acylneuraminate methyl ester
-
 Mannheimia haemolytica A2 diphosphate + CMP-N-acylneuraminate methyl ester
-
 ?
2.7.7.43 CTP + N-glycolylneuraminate
-
 Mannheimia haemolytica diphosphate + CMP-N-glycolylneuraminate
-
 ?
2.7.7.43 TTP + N-acylneuraminate
-
 Mannheimia haemolytica diphosphate + TMP-N-acylneuraminate
-
 ?
2.7.7.43 TTP + N-acylneuraminate
-
 Mannheimia haemolytica A2 diphosphate + TMP-N-acylneuraminate
-
 ?
2.7.7.43 UDP + N-acetylneuraminate
-
 Mannheimia haemolytica phosphate + UMP-N-acetylneuraminate
-
 ?
2.7.7.43 UTP + N-acylneuraminate
-
 Mannheimia haemolytica diphosphate + UMP-N-acylneuraminate
-
 ?
2.7.7.43 UTP + N-acylneuraminate
-
 Mannheimia haemolytica A2 diphosphate + UMP-N-acylneuraminate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.43 trimer 3 * 43000, SDS-PAGE Mannheimia haemolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 9
-
-
 Mannheimia haemolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.43 7 12.5 activity decreases dramatically below pH 7.0 and above pH 12.5 Mannheimia haemolytica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.43 additional information
-
 additional information
-
 Mannheimia haemolytica