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Literature summary extracted from

  • Nakata, D.; Munster, A.K.; Gerardy-Schahn, R.; Aoki, N.; Matsuda, T.; Kitajima, K.
    Molecular cloning of a unique CMP-sialic acid synthetase that effectively utilizes both deaminoneuraminic acid (KDN) and N-acetylneuraminic acid (Neu5Ac) as substrates (2001), Glycobiology, 11, 685-692.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.43 expression in Escherichia coli Oncorhynchus mykiss
2.7.7.43 expression in Escherichia coli Mus musculus
2.7.7.92 expression in Escherichia coli Oncorhynchus mykiss
2.7.7.92 expression in Escherichia coli Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.26
-
 N-acetylneuraminic acid pH 9.0, 25°C Mus musculus
2.7.7.43 0.26
-
 N-acetylneuraminate pH 9.0, 37°C Mus musculus
2.7.7.43 0.56
-
 3-deoxy-D-glycero-D-galacto-nononate pH 9.0, 25°C Mus musculus
2.7.7.43 0.56
-
 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid pH 9.0, 37°C Mus musculus
2.7.7.43 2.8
-
 N-acetylneuraminic acid pH 9.0, 25°C Oncorhynchus mykiss
2.7.7.43 2.8
-
 N-acetylneuraminate pH 9.0, 37°C Oncorhynchus mykiss
2.7.7.43 3
-
 3-deoxy-D-glycero-D-galacto-nononate pH 9.0, 25°C Oncorhynchus mykiss
2.7.7.43 3
-
 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid pH 9.0, 37°C Oncorhynchus mykiss
2.7.7.92 0.26
-
 N-acetylneuraminic acid pH 9.0, 25°C Mus musculus
2.7.7.92 0.56
-
 3-deoxy-D-glycero-D-galacto-nononate pH 9.0, 25°C Mus musculus
2.7.7.92 2.8
-
 N-acetylneuraminic acid pH 9.0, 25°C Oncorhynchus mykiss
2.7.7.92 3
-
 3-deoxy-D-glycero-D-galacto-nononate pH 9.0, 25°C Oncorhynchus mykiss

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.43 66000
-
 SDS-PAGE, recombinant thioredoxin fusion protein Mus musculus
2.7.7.43 69000
-
 x * 69000, SDS-PAGE Oncorhynchus mykiss
2.7.7.43 69000
-
 SDS-PAGE, recombinant thioredoxin fusion protein Oncorhynchus mykiss
2.7.7.92 66000
-
 SDS-PAGE, recombinant thioredoxin fusion protein Mus musculus
2.7.7.92 69000
-
 SDS-PAGE, recombinant thioredoxin fusion protein Oncorhynchus mykiss

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Mus musculus Q99KK2
-
-
2.7.7.43 Oncorhynchus mykiss Q90WG6
-
-
2.7.7.92 Mus musculus Q99KK2 cf. EC 2.7.7.43
-
2.7.7.92 Oncorhynchus mykiss Q90WG6 cf. EC 2.7.7.43
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.43 liver
-
 Oncorhynchus mykiss
-
2.7.7.43 ovary
-
 Oncorhynchus mykiss
-
2.7.7.43 testis
-
 Oncorhynchus mykiss
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid enzyme shows high activity towards 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid and towards N-acetylneuraminate Oncorhynchus mykiss diphosphate + CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononic acid
-
 ?
2.7.7.43 CTP + 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid recombinant enzyme shows 15times lower activity towards 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid than towards N-acetylneuraminate Mus musculus diphosphate + CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononic acid
-
 ?
2.7.7.43 CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Oncorhynchus mykiss diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate reaction of EC 2.7.7.92 ?
2.7.7.43 CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Mus musculus diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate reaction of EC 2.7.7.92 ?
2.7.7.43 CTP + N-acetylneuraminic acid
-
 Oncorhynchus mykiss diphosphate + CMP-N-acetylneuraminic acid
-
 ?
2.7.7.43 CTP + N-acetylneuraminic acid
-
 Mus musculus diphosphate + CMP-N-acetylneuraminic acid
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Oncorhynchus mykiss diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Mus musculus diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.92 CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Oncorhynchus mykiss diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
2.7.7.92 CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
 Mus musculus diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
2.7.7.92 CTP + N-acetylneuraminic acid
-
 Oncorhynchus mykiss diphosphate + CMP-N-acetylneuraminic acid
-
 ?
2.7.7.92 CTP + N-acetylneuraminic acid
-
 Mus musculus diphosphate + CMP-N-acetylneuraminic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.43 ?
-
 Mus musculus
2.7.7.43 ? x * 69000, SDS-PAGE Oncorhynchus mykiss

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.43 25
-
 unstable even at Oncorhynchus mykiss