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Literature summary extracted from

  • Corfield, A.P.; Schauer, R.; Wember, M.
    The preparation of CMP-sialic acids by using CMP-acylneuraminate synthase from frog liver immobilized on sepharose 4B (1979), Biochem. J., 177, 1-7.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.43 2-mercaptoethanol maximal activation, 210%, at 1 mM Pelophylax lessonae

General Stability

EC Number General Stability Organism
2.7.7.43 enzyme is stable in lyophilized form Pelophylax lessonae
2.7.7.43 stability can be improved by 1 mM 2-mercaptoethanol Pelophylax lessonae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 2-deoxy-2,3-dehydro-N-acetylneuraminate 4.6 mM, 33% inhibition Pelophylax lessonae
2.7.7.43 CDP 1 mM, 49% inhibition Pelophylax lessonae
2.7.7.43 CMP 1 mM, 43% inhibition Pelophylax lessonae
2.7.7.43 diphosphate 1 mM, 46% inhibition Pelophylax lessonae
2.7.7.43 NaN3 5 mM, 70% inhibition Pelophylax lessonae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.00036
-
 N-acetylneuraminate pH 9.0, 37°C Pelophylax lessonae
2.7.7.43 0.00046
-
 CTP pH 9.0, 37°C Pelophylax lessonae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Mg2+ 40 mM, 30% stimulation Pelophylax lessonae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Pelophylax lessonae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.43 liver
-
 Pelophylax lessonae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Pelophylax lessonae diphosphate + CMP-N-acylneuraminate
-
 ?