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Literature summary extracted from

  • Rodriguez-Aparicio, L.B.; Luengo, J.M.; Gonzalez-Clemente, C.; Reglero, A.
    Purification and characterization of the nuclear cytidine 5-monophosphate N-acetylneuraminic acid synthetase from rat liver (1992), J. Biol. Chem., 267, 9257-9263.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.43 2-mercaptoethanol 5 mM, activation to 138% of original activity Rattus norvegicus
2.7.7.43 dithiothreitol 5 mM, activates to 193% of the original activity Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.7.43 purified enzyme is sensitive to repeated freezing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 (NH4)2SO4 1.0 M, 83% inhibition, reversible by desalting Rattus norvegicus
2.7.7.43 AMP 10 mM in presence of 3 mM CTP, 43% inhibition Rattus norvegicus
2.7.7.43 CDP 10 mM in presence of 3 mM CTP, 73% inhibition Rattus norvegicus
2.7.7.43 CMP 10 mM in presence of 3 mM CTP, 89% inhibition Rattus norvegicus
2.7.7.43 CMP-N-acylneuraminate 5 mM, 60% inhibition Rattus norvegicus
2.7.7.43 CTP at high concentrations Rattus norvegicus
2.7.7.43 Cu2+ 10 mM, 98% inhibition Rattus norvegicus
2.7.7.43 Fe3+ 10 mM, 50% inhibition Rattus norvegicus
2.7.7.43 GTP 10 mM in presence of 3 mM CTP, 63% inhibition Rattus norvegicus
2.7.7.43 Hg2+ 10 mM, 62% inhibition Rattus norvegicus
2.7.7.43 TTP 10 mM in presence of 3 mM CTP, 71% inhibition Rattus norvegicus
2.7.7.43 UTP 10 mM in presence of 3 mM CTP, 75% inhibition Rattus norvegicus
2.7.7.43 Zn2+ 10 mM, 98% of maximal activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 1.3
-
 N-acetylneuraminate pH 8.0, 37°C Rattus norvegicus
2.7.7.43 1.5
-
 CTP pH 8.0, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.43 nucleus
-
 Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Ca2+ 33% of the activation with Mg2+ Rattus norvegicus
2.7.7.43 Co2+ 80% of the activation with Mg2+ Rattus norvegicus
2.7.7.43 Mg2+ maximal activity at 10 mM Rattus norvegicus
2.7.7.43 Mn2+ 70% of the activity with Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.43 58000
-
 2 * 58000, SDS-PAGE Rattus norvegicus
2.7.7.43 116000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.43 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.43 2
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.7.43 -20°C, 2 weeks, more than 50% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Rattus norvegicus diphosphate + CMP-N-acylneuraminate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.43 dimer 2 * 58000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.43 45
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.43 30 50 30°C: about 40% of maximal activity, 50°C: about 90% of maximal activity Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.43 35
-
 60 min, completely stable Rattus norvegicus
2.7.7.43 37
-
 60 min, about 10% loss of activity Rattus norvegicus
2.7.7.43 40
-
 60 min, 20% loss of activity Rattus norvegicus
2.7.7.43 45
-
 60 min, 65% loss of activity, less than 10% loss of activity in presence of 20% w/v glycerol Rattus norvegicus
2.7.7.43 50
-
 10 min, 85% loss of activity Rattus norvegicus
2.7.7.43 55
-
 20 min, complete loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 8
-
 at an ionic strength ranging between 50-250 mM, in Tris-HCl buffer, glycine-NaOH as well as in phosphate buffer Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.43 7 10 pH 7.0: about 90% of maximal activity, pH 10.0: about 70% of maximal activity Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.43 Rattus norvegicus chromatofocusing
-
 6.5