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Literature summary extracted from

  • Follens, A.; Veiga-da-Cunha, M.; Merckx, R.; van Schaftingen, E.; van Eldere, J.
    acs1 of Haemophilus influenzae type a capsulation locus region II encodes a bifunctional ribulose 5-phosphate reductase- CDP-ribitol pyrophosphorylase (1999), J. Bacteriol., 181, 2001-2007.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.405 CTP marked stimulation, presence of 50 mM CTP decreases the Km value for ribulose 5-phosphate from 400 to 50 microM Haemophilus influenzae
1.1.1.405 additional information not stimulated by UTP, ATP, GTP, ADP, or dCTP Haemophilus influenzae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.405 expression in ERscherichia coli Haemophilus influenzae
2.7.7.40 expressed in Escherichia coli Haemophilus influenzae

General Stability

EC Number General Stability Organism
1.1.1.405 23°C, at 0.3 mg/ml in the presence of 20 mM HEPES, pH 7.1, 1 mM DTT, and 0.5 mg of BSA/ml, ribitol 5-phosphate dehydrogenase activity decreases to about 50% of the initial activity after 2 h Haemophilus influenzae
2.7.7.40 unstable, 50% loss of activity after 2 h at 23°C in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.05 mM CTP Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.405 additional information not inhibitory: 0.5 mM ribitol 5-phosphate or 0.5 mM NADP+ Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.405 0.01
-
 NADPH pH 7.1, 30°C Haemophilus influenzae
1.1.1.405 0.05
-
 D-ribitol 5-phosphate presence of CTP, pH 7.1, 30°C Haemophilus influenzae
1.1.1.405 0.4
-
 D-ribitol 5-phosphate pH 7.1, 30°C Haemophilus influenzae
2.7.7.40 0.037
-
 D-ribitol 5-phosphate pH 7.1, 30°C Haemophilus influenzae
2.7.7.40 0.15
-
 CTP pH 7.1, 30°C Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.405 53000
-
 x * 53000, SDS-PAGE Haemophilus influenzae
2.7.7.40 53000
-
 SDS-PAGE Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.40 CTP + D-ribitol 5-phosphate Haemophilus influenzae
-
 diphosphate + CDPribitol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.405 Haemophilus influenzae Q48154 additionally catalyzes reaction of ribitol-5-phosphate cytidylyltransferase, EC 2.7.7.40
-
2.7.7.40 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.40 enzyme copurifies with ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.405 0.73
-
 substrate D-ribulose 5-phosphate, pH 8.5, 30°C Haemophilus influenzae
1.1.1.405 2.87
-
 substrate D-ribulose 5-phosphate, presence of CTP, pH 8.5, 30°C Haemophilus influenzae
2.7.7.40 15.7
-
 CDP-ribitol pyrophosphorylase activity Haemophilus influenzae
2.7.7.40 37.5
-
 ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.405 D-ribitol 5-phosphate + NADP+
-
 Haemophilus influenzae D-ribulose 5-phosphate + NADPH + H+
-
 r
1.1.1.405 D-ribulose 5-phosphate + NADPH + H+
-
 Haemophilus influenzae D-ribitol 5-phosphate + NADP+
-
 r
1.1.1.405 additional information the dehydrogenase activity of Acs1 is specific for ribulose 5-phosphate and NADPH in one direction and for ribitol 5-phosphate and NADP+ in the other direction. The activity is about twofold higher with ribulose 5-phosphate as the substrate than with ribose 5-phosphate. No substrate: xylulose 5-phosphate Haemophilus influenzae ?
-
 ?
2.7.7.40 CTP + D-arabitol 5-phosphate
-
 Haemophilus influenzae diphosphate + CDParabitol
-
 ?
2.7.7.40 CTP + D-ribitol 5-phosphate
-
 Haemophilus influenzae diphosphate + CDPribitol
-
 ?
2.7.7.40 additional information enzyme has also ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae ?
-
 ?
2.7.7.40 additional information no activity with erythritol 4-phosphate, sorbitol 6-phosphate and UTP Haemophilus influenzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.405 ? x * 53000, SDS-PAGE Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.405 ACS1
-
 Haemophilus influenzae
1.1.1.405 bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase
-
 Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.40 30
-
 assay at Haemophilus influenzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.40 23
-
 50% loss of activity after 2 h in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.005 mM CTP Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.405 7 8.4 broad Haemophilus influenzae
2.7.7.40 7.1
-
 same enzyme activity at pH 7.1 and pH 8 Haemophilus influenzae
2.7.7.40 8
-
 same enzyme activity at pH 7.1 and pH 8 Haemophilus influenzae

General Information

EC Number General Information Comment Organism
1.1.1.405 physiological function Acs1 encodes a bifunctional enzyme that converts ribulose 5-phosphate into ribitol 5-phosphate and further into CDP-ribitol, the activated precursor form for incorporation of ribitol 5-phosphate into the Haemophilus influenzae type a capsular polysaccharide Haemophilus influenzae