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Literature summary extracted from

  • Park, Y.S.; Sweitzer, T.D.; Dixon, J.E.; Kent, C.
    Expression, purification, and characterization of CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis (1993), J. Biol. Chem., 268, 16648-16654.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.39 gene expressed in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.39 Cd2+
-
Bacillus subtilis
2.7.7.39 CDPglycerol
-
Bacillus subtilis
2.7.7.39 Cu2+
-
Bacillus subtilis
2.7.7.39 diphosphate
-
Bacillus subtilis
2.7.7.39 Hg2+
-
Bacillus subtilis
2.7.7.39 Sn2+
-
Bacillus subtilis
2.7.7.39 Zn2+
-
Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.39 3.23
-
glycerol 3-phosphate pH 8, 37°C Bacillus subtilis
2.7.7.39 3.85
-
CTP pH 8, 37°C Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.39 Co2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis
2.7.7.39 Fe2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis
2.7.7.39 Mg2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis
2.7.7.39 Mn2+ divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.39 14800
-
2 * 14800, gene expression in Escherichia coli, SDS-PAGE Bacillus subtilis
2.7.7.39 15271
-
2 * 15271, calculated from the nucleotide sequence Bacillus subtilis
2.7.7.39 30900
-
gene expression in E. coli, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.39 CTP + sn-glycerol 3-phosphate Bacillus subtilis enzyme of teichoic acid synthesis diphosphate + CDPglycerol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.39 Bacillus subtilis
-
BR151, gene expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.39 gene expression in E. coli Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.39 CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol rapid equilibrium random order mechanism Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.39 85.7
-
-
Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.7.7.39 -20°C, about 20% loss of activity after 1 month Bacillus subtilis
2.7.7.39 -80°C, pure enzyme stable for at least 7 months Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.39 CTP + sn-glycerol 3-phosphate
-
Bacillus subtilis diphosphate + CDPglycerol
-
?
2.7.7.39 CTP + sn-glycerol 3-phosphate enzyme of teichoic acid synthesis Bacillus subtilis diphosphate + CDPglycerol
-
?
2.7.7.39 dCTP + glycerol 3-phosphate about 95% of the activity with CTP Bacillus subtilis diphosphate + dCDPglycerol
-
?
2.7.7.39 additional information no substrate: GTP Bacillus subtilis ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.39 dimer 2 * 14800, gene expression in Escherichia coli, SDS-PAGE Bacillus subtilis
2.7.7.39 dimer 2 * 15271, calculated from the nucleotide sequence Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.39 37
-
assay at Bacillus subtilis
2.7.7.39 50
-
-
Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.39 40
-
not stable for longer than 30 min Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.39 6.9 9.5
-
Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.39 6.5
-
37°C, 4 h, about 30% loss of activity Bacillus subtilis
2.7.7.39 7.5 9.5 37°C, 4 h, stable Bacillus subtilis
2.7.7.39 10
-
37°C, 4 h, about 10% loss of activity Bacillus subtilis