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Literature summary extracted from

  • Munier-lehmann, H.; Chaffotte, A.; Pochet, S.; Labesse, G.
    Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes (2001), Protein Sci., 10, 1195-1205.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.9 overexpression in Escherichia coli Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
2.7.4.9 dissociation of the native dimeric species of the enzyme occurs at an urea concentration of around 4 M, leading to the accumulation of partially folded monomers that unfold totally at urea concentrations above 5.5 M Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 3'-azido-3'-deoxythymidine 5'-monophosphate potent competitive inhibitor Mycobacterium tuberculosis
2.7.4.9 3'-azido-3'-deoxythymidine monophosphate
-
 Mycobacterium tuberculosis
2.7.4.9 5-methyl iso-dCMP competitive Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.0045
-
 dTMP pH 7.4, 30°C Mycobacterium tuberculosis
2.7.4.9 0.1
-
 ATP pH 7.4, 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.9 22635
-
 2 * 22635, electrospray ionization mass spectrometry Mycobacterium tuberculosis
2.7.4.9 46600
-
 sedimentation equilibrium ultracentrifugation Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + dTMP
-
 Mycobacterium tuberculosis ADP + dTDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.9 dimer 2 * 22635, electrospray ionization mass spectrometry Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.9 65
-
 10 min, 50% inactivation, irreversible Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.02
-
 3'-azido-3'-deoxythymidine monophosphate pH 7.4, 30°C Mycobacterium tuberculosis
2.7.4.9 0.13
-
 5-methyl iso-dCMP pH 7.4, 30°C Mycobacterium tuberculosis