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Literature summary extracted from

  • Cheng, Y.C.; Prusoff, W.H.
    Mouse ascites sarcoma 180 thymidylate kinase. General properties, kinetic analysis, and inhibition studies (1973), Biochemistry, 12, 2612-2619.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.9 the enzyme is very unstable and may be stabilized in the presence of 2-mercaptoethanol with dTMP, dTDP, or ADP but not with ATP, MgATP2- or mercaptoethanol alone Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 5'-aminodeoxythymidine
-
Mus musculus
2.7.4.9 5'-carboxyldeoxythymidine
-
Mus musculus
2.7.4.9 5'-chlorodeoxythymidine
-
Mus musculus
2.7.4.9 5'-iododeoxythymidine
-
Mus musculus
2.7.4.9 ADP
-
Mus musculus
2.7.4.9 dTTP potent feedback inhibitor of dTMP Mus musculus
2.7.4.9 TDP
-
Mus musculus
2.7.4.9 thymidine
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 1.1
-
MgdATP2-
-
Mus musculus
2.7.4.9 1.5
-
ATP pH 7.5, 37°C Mus musculus
2.7.4.9 1.5
-
ATP ATP in form of MgATP2- Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.9 33000
-
sucrose density gradient centrifugation Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9 partial Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.9 S-180 cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.9 0.024
-
-
Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.4.9 -70°C, 1 week without significant loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + dTMP
-
Mus musculus ADP + dTDP
-
?
2.7.4.9 CTP + dTMP no activity with CTP Mus musculus CDP + dTDP
-
?
2.7.4.9 dATP + dTMP
-
Mus musculus dADP + dTDP
-
?
2.7.4.9 dCTP + dTMP no activity with dCTP Mus musculus dCDP + dTDP
-
?
2.7.4.9 dGTP + dTMP no activity with dGTP Mus musculus dGDP + dTDP
-
?
2.7.4.9 GTP + dTMP no activity with GTP Mus musculus GDP + dTDP
-
?
2.7.4.9 UTP + dTMP no activity with UTP Mus musculus UDP + dTDP
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.9 41
-
10 min, inactivation when dTMP concentration is below 0.05 mM Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.02
-
5'-chlorodeoxythymidine pH 7.5, 37°C Mus musculus
2.7.4.9 0.027
-
dTTP pH 7.5, 37°C Mus musculus
2.7.4.9 0.03
-
dTDP pH 7.5, 37°C Mus musculus
2.7.4.9 0.03
-
5'-carboxyldeoxythymidine pH 7.5, 37°C Mus musculus
2.7.4.9 0.13
-
5'-aminodeoxythymidine pH 7.5, 37°C Mus musculus