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Literature summary extracted from

  • Pilloff, D.; Dabovic, K.; Romanowski, M.J.; Bonanno, J.B.; Doherty, M.; Burley, S.K.; Leyh, T.S.
    The kinetic mechanism of phosphomevalonate kinase (2003), J. Biol. Chem., 278, 4510-4515.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.2 5-diphosphomevalonate
-
 Streptococcus pneumoniae
2.7.4.2 ADP
-
 Streptococcus pneumoniae
2.7.4.2 AMP-PNP competitive dead-end inhibition vs. ATP Streptococcus pneumoniae
2.7.4.2 ATP
-
 Streptococcus pneumoniae
2.7.4.2 mevalonate competitive vs. phosphomevalonate, noncompetitive vs. ATP Streptococcus pneumoniae
2.7.4.2 phosphomevalonate
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.2 0.0042
-
 phosphomevalonate pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 0.012
-
 diphosphomevalonate pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 0.074
-
 ATP pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 0.35
-
 ADP pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 0.38
-
 ADPbetaS pH 7.0, 25°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate Streptococcus pneumoniae enzyme of mevalonate pathway ADP + 5-diphosphomevalonate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.2 Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.2 ADPbetaS + 5-diphosphomevalonate 41fold slower kcat than with ADP Streptococcus pneumoniae ATPbetaS + 5-phosphomevalonate
-
 r
2.7.4.2 ATP + (R)-5-phosphomevalonate enzyme of mevalonate pathway Streptococcus pneumoniae ADP + 5-diphosphomevalonate
-
 r
2.7.4.2 ATP + 5-phosphomevalonate
-
 Streptococcus pneumoniae ADP + 5-diphosphomevalonate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.2 0.095
-
 ADPbetaS pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 3.4
-
 ATP pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 3.9
-
 ADP pH 7.0, 25°C Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.2 0.0077
-
 phosphomevalonate pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 0.014
-
 5-diphosphomevalonate pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 0.137
-
 ATP pH 7.0, 25°C Streptococcus pneumoniae
2.7.4.2 0.41
-
 ADP pH 7.0, 25°C Streptococcus pneumoniae